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pcDNA4TO-24xGCN4_v4-kif18b

Plasmid

#74934

Purpose

Translation reporter that lacks PP7 sites in the 3'UTR

Depositor

Marvin Tanenbaum

Publication

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

24xGCN4_v4-kif18b

Expression

Mammalian

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJA291

Plasmid

#74487

Purpose

eF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFP

Depositor

Andrew Fire

Publication

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

puroR-mCherry-T2A-eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GST-PRDM16 880-1038

Plasmid

#53350

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Publication

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 880-1038

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 224-454

Plasmid

#53347

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Publication

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 224-454

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
pRK5-mWnt2

Plasmid

#42274

Depositor

Chris Garcia, Jeremy Nathans

Publication

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Wnt2 (Wnt2 Mouse)

Expression

Mammalian

Available since

June 21, 2013

Availability

Academic Institutions and Nonprofits only
pGEX 4T1 RARalpha

Plasmid

#35557

Depositor

Jonathan Kurie, Harish Srinivas

Publication

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Tags

GST

Expression

Bacterial

Available since

April 5, 2012

Availability

Academic Institutions and Nonprofits only
CD200Cd4d3+4-bio

Plasmid

#32404

Purpose

A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Publication

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD200

Tags

Cd4d3+4 and biotinylation peptide

Expression

Mammalian

Promoter

CMV

Available since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCold TDP43 G294A

Plasmid

#27452

Depositor

Aaron Gitler

Publication

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

Factor Xa and His6

Expression

Bacterial

Mutation

Glycine 294 mutated to Alanine (G294A)

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pB1H2w5-12-C81S

Plasmid

#18043

Depositor

Scot Wolfe

Publication

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

HhaI C81S mutant

Expression

Bacterial

Mutation

Simply used as an insert between Kpn1 and Xba1

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pMDH

Plasmid

#133129

Purpose

A recombinant plasmid expressing mdh (malate dehydrogenase) gene

Depositor

Gregg Beckham

Publication

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

malate dehydrogenase gene

Expression

Bacterial

Promoter

malate dehydrogenase promoter

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR221_SLC7A8

Plasmid

#132233

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A8 (SLC7A8 Human)

Expression

Mammalian

Available since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pMLBAD-eGFP

Plasmid

#112918

Purpose

Fluorescence microscopy

Depositor

Miguel Valvano

Publication

Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

Insert

eGFP

Expression

Bacterial

Promoter

Pbad

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pIRESpuroN3xFLAG-UFSP2

Plasmid

#204553

Purpose

Expresses 3xFLAG-tagged UFSP2 in mammalian cells

Depositor

Masaaki Komatsu

Publication

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFSP2 (UFSP2 Human)

Expression

Mammalian

Mutation

none

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pIA1400

Plasmid

#172519

Purpose

Expression of SARS-COV-2 Nsp12 in E. coli

Depositor

Irina Artsimovitch

Publication

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mito-mEmerald

Plasmid

#174542

Purpose

labels mitochondria with mEmerald

Depositor

Minna Roh-Johnson

Publication

Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

Insert

MTS-mEmerald

Use

Lentiviral

Tags

mEmerald

Expression

Mammalian

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1a-mCherryLuciferase-W

Plasmid

#200101

Purpose

Lentiviral vector expressing mCherry fused with Luciferase at the C-terminus

Depositor

Kosuke Yusa

Publication

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Luciferase

Use

CRISPR and Lentiviral

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSH124-ssr

Plasmid

#198025

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Publication

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Ap/Cb resistance cassette

Expression

Bacterial

Promoter

ApR promoter

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-QUE2m

Plasmid

#129308

Purpose

QUEEN ATP sensor for 25degC

Depositor

Yasushi Okada

Publication

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

QUEEN-2m

Expression

Mammalian

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z07

Plasmid

#124790

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreen

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p CIneo-RL

Plasmid

#115366

Purpose

Expression vector to produce humanized Renilla luciferase

Depositor

Witold Filipowicz, Ramesh Pillai

Publication

Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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