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FP5369 KIF17MD-flag- FIREmate

Plasmid

#216724

Purpose

Expresses motor domain of KIF17 fused to FIREmate in mamallian cells

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

KIF17 (KIF17 Human)

Use

Tags

FIREmate and Flag

Expression

Mammalian

Mutation

1-547

Promoter

CMV

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria

Plasmid

#44251

Purpose

Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA (bacteria)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 (SpeI)

Available since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

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mRuby3-C1

Plasmid

#127808

Purpose

mRuby3 C1 Cloning vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mRuby3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mito-mEmerald

Plasmid

#174542

Purpose

labels mitochondria with mEmerald

Depositor

Minna Roh-Johnson

Article

Kidwell et al Elife. 2023 Mar 6;12:e85494. doi: 10.755

Insert

MTS-mEmerald

Use

Lentiviral

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-hMLH1dn for IVT

Plasmid

#178114

Purpose

Template for in vitro transcription of human MLH1dn

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized) (MLH1 Human)

Use

Template for in vitro transcription

Tags

Expression

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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EDNRA-DuET

Plasmid

#213232

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

EDNRA (EDNRA Human)

Use

Tags

1D4 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PARP1

Plasmid

#211578

Purpose

CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

Plasmid

#138460

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-KRAB-IRES-zsGreen1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CMVdel-dimericTomato-WASp(CRIB)

Plasmid

#191450

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WASp

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-CamKII-TRPV1-P2A-mCherry

Plasmid

#200829

Purpose

Transduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporter

Depositor

Hong Chen

Article

Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

Insert

TRPV1-P2A-mCherry (Trpv1 Rat)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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