Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 20,388 results for: ACE;

Showing: 9751 - 9780 of 20388 results

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pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(S17N)-GID1-mVenus-CAAX

Plasmid

#232263

Purpose

Express split-KRas fragments that are fused with CIDs and FPs

Depositor

Cliff Stains

Article

He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

Inserts

mCerulean-GAI1-92-KRas/N12 (KRAS Human)
KRas/13C(S17N)-GID1-mVenus-CAAX (KRAS Human)

Tags

mCerulean and mVenus

Expression

Mammalian

Mutation

KRas-S17N

Promoter

CMV

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6f

Plasmid

#182548

Purpose

Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Expression

Bacterial and Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80L-His-SpyTag-XMod-Doc(WT)

Plasmid

#157675

Purpose

Expression of XMod-Doc wild type with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

XMod-Doc Wild type with Histag and SpyTag

Tags

Histag and SpyTag

Expression

Bacterial

Promoter

T5

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L-Coh-His-SpyTag

Plasmid

#157673

Purpose

Expression of Coh (type III from Ruminococcus flavefaciens) with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

Cohesin (R.f) with Histag and SpyTag

Tags

Histag and SpyTag

Expression

Bacterial

Promoter

T5

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-SQSTM1-WT

Plasmid

#194582

Purpose

Mammalian Expression of mEGFP-SQSTM1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

SQSTM1 (SQSTM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-Full-length

Plasmid

#194550

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a-mCherry-MED1-IDR

Plasmid

#194545

Purpose

Bacterial Expression of 6xHis mCherry-MED1-IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

MED1 (MED1 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-DVL1-WT

Plasmid

#194586

Purpose

Mammalian Expression of mEGFP-DVL1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

DVL1 (DVL1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-WT-IDR

Plasmid

#194549

Purpose

Mammalian Expression of mEGFP-HMGB1 WT IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET-45b-mEGFP-HMGB1-WT

Plasmid

#194543

Purpose

Bacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-WT-Full-length

Plasmid

#194548

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-RUNX1-WT

Plasmid

#194574

Purpose

Mammalian Expression of mEGFP-RUNX1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

RUNX1 (RUNX1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-SOX2-WT

Plasmid

#194580

Purpose

Mammalian Expression of mEGFP- SOX2 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

SOX2 (SOX2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-HMGB1-WT

Plasmid

#194561

Purpose

Dox-inducible Mammalian Expression of mEGFP-HMGB1 WT IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-CALR-WT

Plasmid

#194578

Purpose

Mammalian Expression of mEGFP-CALR WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

CALR (CALR Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB3-WT

Plasmid

#194564

Purpose

Mammalian Expression of mEGFP-HMGB3 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB3 (HMGB3 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-IDR2

Plasmid

#194552

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB3-MUT

Plasmid

#194565

Purpose

Mammalian Expression of mEGFP-HMGB3 Mutant (K161Ifs*55) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB3 (HMGB3 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

K161Ifs*55

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-FOXC1-MUT

Plasmid

#194567

Purpose

Mammalian Expression of mEGFP-FOXC1 Mutant (Q200Rfs*109) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXC1 (FOXC1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

Q200Rfs*109

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-FOXC1-WT

Plasmid

#194566

Purpose

Mammalian Expression of mEGFP-FOXC1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXC1 (FOXC1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-delIDR

Plasmid

#194554

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein, "del IDR" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

truncation by stop codon after Asn134

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-FOXF1-WT

Plasmid

#194568

Purpose

Mammalian Expression of mEGFP-FOXF1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXF1 (FOXF1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-IDR

Plasmid

#194551

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-FOXL2-WT

Plasmid

#194584

Purpose

Mammalian Expression of mEGFP-FOXL2 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXL2 (FOXL2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-FOXF1-MUT

Plasmid

#194569

Purpose

Mammalian Expression of mEGFP-FOXF1 Mutant (A231Rfs*61) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXF1 (FOXF1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

A231Rfs*61

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-FOXL2-MUT

Plasmid

#194585

Purpose

Mammalian Expression of mEGFP-FOXL2 Mutant (A328Pfs*28) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXL2 (FOXL2 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

A328Pfs*28

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (JN.1)

Plasmid

#241117

Purpose

Expresses SARS-CoV-2 spike (JN.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (JN.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

ins16MPLF,T19I,R21T,L24del,P25del,P26del,A27S,S50…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.86)

Plasmid

#241090

Purpose

Expresses SARS-CoV-2 spike (BA.2.86) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.86) (S SARS-CoV-2)

Expression

Mammalian

Mutation

ins16MPLF, T19I, R21T, L24del, P25del, P26del, A2…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-delFS

Plasmid

#194555

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein, "del FS" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

truncation by stop codon after Lys183

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-DVL1-MUT

Plasmid

#194587

Purpose

Mammalian Expression of mEGFP-DVL1 Mutant (H502Pfs*143) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

DVL1 (DVL1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

H502Pfs*143

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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