We narrowed to 7,672 results for: CCH

1. pCOLA-Gent-EM7-Erv1p-PDI

   Plasmid

   #202486

   Purpose

   Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)
   

   Expression

   Bacterial

   Promoter

   EM7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TRE-ChR2-YFP

   Plasmid

   #171622

   Purpose

   TRE-ChR2-YFP reporter

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   TRE-ChR2-YFP
   

   Use

   AAV and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TetO

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLM494

   Plasmid

   #100539

   Purpose

   CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.

   Depositor

   Bernd Müller-Röber

   Article

   Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

   Inserts

   GGPP synthase
   phytoene desaturase
   phytoene-beta carotene synthase
   HMG CoA reductase
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Yeast

   Promoter

   PGK1, TDH3, TIP1, and ZEO1

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15b His-p97

   Plasmid

   #169020

   Purpose

   Expresses His-p97 in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGal4AD-CIB1

   Plasmid

   #28245

   Purpose

   Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   Gal4AD (CIB1, GAL4 Budding Yeast)
   

   Tags

   CIB1

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTW040

   Plasmid

   #64769

   Purpose

   For integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminator

   Depositor

   Fred van Leeuwen

   Article

   Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

   Insert

   Cre-EBD78
   

   Tags

   fused to the human Estrogen Binding Domain (EBD),…

   Expression

   Yeast

   Promoter

   TDH3

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBMN-HA-TBK1

   Plasmid

   #199205

   Purpose

   Used for the expression of TBK1 (SMC Internal No. 1996)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYES260-sc.eEF2 K509A

   Plasmid

   #85120

   Purpose

   yeast expression of mutant EEF2

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   K509A

   Promoter

   GAL1

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBbsr2-4031NES

   Plasmid

   #105241

   Purpose

   A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.

   Depositor

   Michiyuki Matsuda

   Article

   Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

   Insert

   AMPK-EV
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBMN HA-NDP52

    Plasmid

    #199307

    Purpose

    For expression of the mammalian NDP52 tagged with HA

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAEL010097-Cas9

    Plasmid

    #100707

    Purpose

    The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL010097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Promoter

    AAEL010097 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA

    Plasmid

    #155125

    Purpose

    Tol2 vector containing Cre-inducible QFGal4-switch

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QFGal4-switch
    

    Use

    Cre/Lox; Zebrafish expression

    Promoter

    Ubi

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SNAP-OMP25

    Plasmid

    #141149

    Purpose

    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Budding Yeast, Rat)
    

    Tags

    SNAP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCD510B attp BSD

    Plasmid

    #216236

    Purpose

    Lentiviral vector, For genome integration of an attP PhiC13 recognition site

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Insert

    blasticidin S deaminase
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. MBP-TSF-TEVcs-ULK1

    Plasmid

    #171416

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ULK1 (ULK1 Synthetic)
    

    Expression

    Mammalian

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTP-PCR C3

    Plasmid

    #189620

    Purpose

    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

    Insert

    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    

    Use

    Synthetic Biology; Diatom expression

    Expression

    Bacterial and Yeast

    Mutation

    Removed repeat region, 10-kbp deletion, and minor…

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEasyG2_mic

    Plasmid

    #184916

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMC234r-ccdB/TPK2

    Plasmid

    #176179

    Purpose

    yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    dropout cassette containing toxic genes ccdB and TPK2
    

    Use

    Synthetic Biology

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

    Plasmid

    #86421

    Purpose

    N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    CDC42pr-KAR2SS-GFP1-10
    

    Tags

    GFP1-10

    Expression

    Yeast

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-hPGK-eGFP-SynaptoZip

    Plasmid

    #122523

    Purpose

    Lentiviral expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by hPGK promoter

    Depositor

    Antonio Malgaroli

    Article

    Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

    Insert

    eGFP-SynaptoZip (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    Myc and eGFP

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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