We narrowed to 10,454 results for: ESP

1. pDN-G1TGt

   Plasmid

   #44508

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
   tetR::yEGFP
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Expression

   Yeast

   Mutation

   Two tandem tet operators (2XtetO2) downstream of …

   Promoter

   PGal1-D12 promoter

   Available Since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. p426_Cas9_gRNA-HIS3b

   Plasmid

   #87402

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting HIS3b sequence AATATAGAGTGTACTAGAGG in yeast chromosome 15.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting HIS3b
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet

   Plasmid

   #176535

   Purpose

   Non-protein coding locus components from CRISPR-associated IscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   non-coding locus components from Delaware Bay metagenome CRISPR-associated IscB
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p426_Cas9_gRNA-ARS1309a

   Plasmid

   #87399

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1309a sequence CCTGTGGTGACTACGTATCC in yeast chromosome 13.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1309a
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRESneo-MPG(N169D)

   Plasmid

   #23259

   Depositor

   Robert Sobol

   Article

   Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

   Insert

   N-methylpurine DNA glycosylase (N169D) (MPG Human)
   

   Expression

   Mammalian

   Mutation

   N169D

   Available Since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

6. psicheck-2-LPPRC-mod

   Plasmid

   #48168

   Purpose

   contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   LRPPRC leucine rich pentatricopeptide repeat containing (LRPPRC Human)
   

   Use

   Luciferase

   Mutation

   A/T mutation in the ATG of the uORF

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

7. p426_Cas9_gRNA-ARS1622b

   Plasmid

   #87403

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1622b sequence GTCACGTTCCTGAGGTTACT in yeast chromosome 16.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1622b
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-HA p21 K161R, K163R

   Plasmid

   #78789

   Purpose

   To overexpress p21 K161R, K163R in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

   Insert

   p21 (CDKN1A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K161R, K163R_muation

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCin4-3xFLAG-MKL1 S449A/T450A/S454A

   Plasmid

   #19845

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S449A/T450A/S454A (MRTFA Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 449, Threonine 450 and Serine 454 …

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5/BirA/FLAG-DDX54

    Plasmid

    #97063

    Purpose

    Expresses BirA/FLAG-tagged DDX54 in mammalian cells

    Depositor

    Markus Landthaler

    Article

    Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

    Insert

    DDX54 (DDX54 Human)
    

    Tags

    BirA and FLAG

    Expression

    Mammalian

    Promoter

    tetO2

    Available Since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST27-GST-ATXN1 FL(92Q)-S776D

    Plasmid

    #21757

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 92 Q(Glutamines) and…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pCS3-MT-BX-6MYC p21

    Plasmid

    #78780

    Purpose

    To overexpress p21 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

    Insert

    p21 (CDKN1A Human)
    

    Tags

    6xMYC

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pEGFP-RNF8-Middle-part

    Plasmid

    #64672

    Purpose

    Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    ring finger protein 8 (RNF8 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Containing only middle part of RNF8 amino acids 1…

    Promoter

    CMV

    Available Since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

    Plasmid

    #176588

    Purpose

    Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    CRISPR-associate IscB (Delaware Bay) locus
    

    Expression

    Bacterial

    Mutation

    Replaced spacer in CRISPR array with Fn spacer

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG71 BDNF 3'UTR mut

    Plasmid

    #12054

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    BDNF 3'UTR mut (BDNF Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDN-T1TCbt

    Plasmid

    #44514

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
    tetR::ymcherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Three tandem tet operators (3XtetO2) downstream o…

    Promoter

    PGal1-T123 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDN-T1GZmbh

    Plasmid

    #44522

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
    yEGFP::ZeoR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Three tandem tet operators (3XtetO2) downstream o…

    Promoter

    PGal1-T123 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p426_Cas9_gRNA-ARS911b

    Plasmid

    #87394

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS911b sequence GTAATATTGTCTTGTTTCCC in yeast chromosome 9.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS911b
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pαH-S-HRV3C/D614G

    Plasmid

    #164568

    Purpose

    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-HRV3C-D614G variant)
    

    Tags

    2X Strep-Tag II and 8X His tag

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_Hygro(+)_GRHL1-L3F6H

    Plasmid

    #177765

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Expression

    Mammalian

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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