We narrowed to 11,813 results for: pcdna

1. 5'-3xFLAG Chtop-exon minimal CMV pCDNA5

   Plasmid

   #212080

   Purpose

   LR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   Chtop-exon (Chtop Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

   Plasmid

   #72888

   Purpose

   fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

   Insert

   Gaussia luciferase, Channelrhodopsin-2, EYFP
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.2 mir-1-1 reporter hsa-mir-122

   Plasmid

   #46673

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-mir-122 (MIR122 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.1(-) rat C/EBP beta (LAP)

   Plasmid

   #12558

   Depositor

   Peter Johnson

   Insert

   CCAAT/Enhancer-binding Protein beta (Cebpb Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3_SARS-CoV-Spike[SARS-CoV-2 RBD]

   Plasmid

   #190086

   Purpose

   express chimeric SARS-CoV spike with the SARS-CoV-2 receptor binding domain

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

   Insert

   SARS-CoV S (S Synthetic)
   

   Use

   Tags

   Rhodopsin C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-iGABASnFR

   Plasmid

   #187091

   Purpose

   GABA sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Pre-SPHERE-iGABASnFR
   

   Use

   Tags

   TagBFP-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-iRFP-P2A-Post-SPHERE-iGABASnFR

   Plasmid

   #187092

   Purpose

   GABA sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Post-SPHERE-iGABASnFR
   

   Use

   Tags

   iRFP670-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127315

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-iRFP-P2A-Post-SPHERE-SF-iGluSnFR

   Plasmid

   #187090

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Post-SPHERE-SF-iGluSnFR
   

   Use

   Tags

   iRFP670-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Dakap-Venus-cpVenus-FLARE-AKAR

    Plasmid

    #123338

    Purpose

    Mitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    DAKAP-Venus-cpVenus-FLARE-AKAR
    

    Use

    Tags

    N-terminal 30 amino acids from DAKAP1, Venus, and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Nrf1-Myc pcDNA3.1(+)-C-Myc (NAWLVH106-AAAAAA)

    Plasmid

    #169126

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hNrf1 (NFE2L1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NAWLVH106-AAAAAA

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

    Plasmid

    #175521

    Purpose

    Nuclear expression of HaloTag10 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 Flag FKHR H215R AAA mutant

    Plasmid

    #13510

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    FKHR H215R AAA (FOXO1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H215R T24A S256A S319A

    Promoter

    Available since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

    Plasmid

    #175421

    Purpose

    Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His

    Plasmid

    #83453

    Purpose

    Full-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    polycystin-1 (Pkd1 Mouse)
    

    Use

    Tags

    2xMyc, 3xFlag, and 6xHis

    Expression

    Mammalian

    Mutation

    S4144D

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

    Plasmid

    #194750

    Purpose

    To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. 9E10.mCHOP(S78&81A).pCDNA1/Amp

    Plasmid

    #21902

    Depositor

    David Ron

    Insert

    CHOP (Ddit3 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S78A S81A

    Promoter

    Available since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

    Plasmid

    #181814

    Purpose

    Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    Muc5b D1 (Muc5b Mouse)
    

    Use

    Tags

    TEV cleavage site followed by 6xHistidine tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

    Plasmid

    #70668

    Purpose

    Human mutant of E2F1 lacking amino acids 2 to 126

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 lacking residues 2 to 126 (E2F1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    lacks amino acids 2-126

    Promoter

    cmv

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

20. 1129 pcDNA3 GFP FKHR H215R AAA

    Plasmid

    #9034

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR H215R AAA (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H215R T24A S256A S319A

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only