We narrowed to 16,131 results for: form

1. Flag-ATXN1_R665A

   Plasmid

   #48195

   Purpose

   Expresses R665A mutated Ataxin1

   Depositor

   Huda Zoghbi

   Article

   Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

   Insert

   Ataxin1 (ATXN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJEC626

   Plasmid

   #174367

   Purpose

   Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.

   Depositor

   James Chappell

   Article

   Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

   Insert

   Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23106

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. hNedd4-2 CS

   Plasmid

   #86041

   Purpose

   full length NeddL3 with C to S mutation with His Xpress tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299

   Insert

   hNeddL3 with C to S mutation in HECT domain (NEDD4 Human)
   

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   C942S mutation in HECT domain

   Promoter

   CMV

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. A337C

   Plasmid

   #24432

   Depositor

   Ron Vale

   Article

   Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

   Insert

   Kinesin (KIF5B Human)
   

   Tags

   6XHis and GFP

   Expression

   Bacterial

   Mutation

   C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C

   Available Since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-MA-VN

   Plasmid

   #123283

   Purpose

   Mammalian expression plasmid for matrix domain of HIV-1 Gag fused to the N-terminal half of split fluorescent Venus (VN)

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   Matrix
   

   Tags

   VN: N-terminus of split Venus (a.a. V1–A154) I152…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGSTag Flag p38 gamma

   Plasmid

   #20818

   Depositor

   Roger Davis

   Article

   Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

   Insert

   pGSTag Flag p38 gamma (Mapk12 Rat)
   

   Tags

   Flag

   Expression

   Bacterial

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pCellFree_G03 FYN

   Plasmid

   #67106

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   FYN (FYN Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. p63RhoGEF580-mVenus-N1

   Plasmid

   #84338

   Purpose

   Expresses p63 (580 a.a.) tagged with mVenus

   Depositor

   Dorus Gadella

   Article

   van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

   Insert

   p63RhoGEF (580 aa) (ARHGEF25 Human)
   

   Tags

   mVenus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLyGo-Kp-2

   Plasmid

   #163144

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. XE158 GFP-delta LZ-XDpr-CS2+

    Plasmid

    #16764

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    delta LZ-XDpr (dact1-b Frog)
    

    Use

    Xenopus expression

    Tags

    gfp

    Mutation

    XDpr is truncated to delete the first 129 amino a…

    Available Since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pGWB422

    Plasmid

    #74816

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pProvector_AAV2_586_PLGLAR

    Plasmid

    #129024

    Purpose

    Recombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position 586.

    Depositor

    Junghae Suh

    Article

    Tong et al J Control Release. 2019 Jun 25. pii: S01

    Insert

    Rep2/Cap2 with PLGLAR lock at 586
    

    Use

    AAV

    Mutation

    inserted peptide motif (PLGLARGDDDDGPLGLAR) at po…

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CaMKII-Mac-GFP

    Plasmid

    #58849

    Purpose

    AAV expression of Mac-GFP under the CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

    Insert

    Mac-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB428

    Plasmid

    #74822

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. actc1b-mKate2-LactC2

    Plasmid

    #109499

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-mKate2-LactC2
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mSTIM1_1-310-CFP

    Plasmid

    #69551

    Purpose

    Expresses a STIM1_1-310 fragment mammalian cells

    Depositor

    Yubin Zhou

    Article

    Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

    Insert

    mSTIM1_1-310
    

    Tags

    Cyan Fluorescent Protein (CFP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCellFree_G03 CREBZF

    Plasmid

    #67070

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CREBZF (CREBZF Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pLenti CRISPR sgSHMT1_2

    Plasmid

    #106312

    Purpose

    Express Cas9 and sgRNA targeting SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. LC402: pMVP (L3-L2) eGFP + polyA

    Plasmid

    #121763

    Purpose

    pMVP L3-L2 entry plasmid, contains eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eGFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. actc1b-mKate2-rab7a

    Plasmid

    #109655

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab7a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab7a (rab7a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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