We narrowed to 18,247 results for: multi
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Plasmid#200661PurposeConstruct for chemogenetically activating and visualizing subpopulations of neurons in the retinaDepositorInsertDREADD Gq-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianPromoterhSynAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-S5E2-FingR-PSD95-mGL
Plasmid#217533PurposeGenetically encoded fluorescent probe for labelling endogenous excitatory synapses on PV interneurons in vivoDepositorPublicationInsertZnF-FingR-PSD95-mGreenLantern (Dlg4 Mouse)
UseAAVTagsmGreenLanternExpressionMammalianPromoterS5E2 enhancerAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SARS-CoV-2-Nsp3PLpro
Plasmid#204208PurposeMammalian expression of SARS-CoV-2 Nsp3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
TagsFLAGx1ExpressionMammalianMutationPLpro of Nsp3Available sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBFC0625
Plasmid#186691PurposeVcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargoDepositorInsertstnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_lacZ_α_1 Spacer crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
ExpressionBacterialPromoterPLacAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pALS099 (pGEX-2TP-cTevHis-DEST)
Plasmid#139787PurposeGateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialAvailable sinceApril 23, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPPEDs
Plasmid#162469Purposeprotoplast test vector of pPPEDDepositorInsertsCas9H840A
attR gateway destination cassette
UsePrime editing destination vector for transient ex…TagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to AlanineAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP
Plasmid#169333PurposeExpression of HaloTag9 at the mitochondrial surface of mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTOMM20-HaloTag9 (TOMM20 Human)
TagsHaloTag9ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas12f-GE ver3.0
Plasmid#176508PurposeExpresses Cas12f-GE in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas12f-GE ver3.0
Cas12f-GE ver3.0
ExpressionMammalianPromoterU6 and chicken β-actinAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + filler DNA reporter plasmid
Plasmid#156454PurposeA mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.DepositorInsertsNeoR/KanR
TcR
ExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7 and tetAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST
Plasmid#127312PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnanoLuciferase
Tags3xFLAG and PESTExpressionMammalianPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA
Plasmid#113686PurposeAn EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.DepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Cre/LoxTagsKRABAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-GABA(B)R2 [N81/37.1R]
Plasmid#114544PurposeMammalian Expression Plasmid of anti-GABA(B)R2 (Human). Derived from hybridoma N81/37.1.DepositorInsertanti-GABA(B)R2 (Homo sapiens) recombinant mouse monoclonal antibody (GABBR2 Mouse)
ExpressionMammalianPromoterdual CMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-baPrs-hdNadV
Plasmid#91950PurposepET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyiDepositorInsertsPutative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases
ExpressionBacterialMutationchanged Leucine 135 to IsoleucinePromoterT7Available sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
LacI INCENP
Plasmid#45236PurposeTarget INCENP to lac operator sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLacI, INCENP-Δcen, mCherry (lacI )
TagsLacI (lac repressor protein) and mCherryExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)Promotert7Available sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR- flag-Lag16-NCAM-1
Plasmid#205199PurposeThis vector encodes of the synCAM (NCAM1) and GFP nanobody LAG16DepositorInsertsynCAM NCAM 1 and Lag16 anti-GFP nanobody
UseLentiviralExpressionMammalianAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGLuc
Plasmid#135288PurposeGBPart - GLuc Luciferase CDSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGaussia Luciferase
UseLuciferase and Synthetic BiologyAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG
Plasmid#139961PurposeOver-expression of heterologus squalene epoxidase from Nannochloropsis oceanicaDepositorInsertConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2
UseSynthetic BiologyAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGrRenilla
Plasmid#118060PurposeGBPart - GreenRenilla Luciferase CDSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGreen Renilla
UseLuciferase and Synthetic Biology; GbpartAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
LoxP1-Mb2YFP-2A (SO244)
Plasmid#99613PurposeTo clone gene of interest downstream of LoxP1-Mb2YFP-2A cassetteDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMb2EYFP
TagsT2AExpressionMammalianAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-BRX-eGFP-NLS
Plasmid#217534PurposeAAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivoDepositorPublicationInsertBMP reporter element and miniXon casette (Smad1 Mouse)
UseAAVExpressionMammalianPromoterBMP Reporter ElementAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only