We narrowed to 10,337 results for: plasmids 101

1. RNF130 shRNA

   Plasmid

   #225340

   Purpose

   shRNAmir backbone for RNA interference, with YFP

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   RNF130 shRNA (Rnf130 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBA

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLN492 (FuGW-G8p-antiPD1(HC)-1Pe)

   Plasmid

   #105194

   Purpose

   Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab heavy chain transcripts containing miR1-Bs(Pe)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   G8p-antiPD1(HC)-1Pe
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLN493 (FuGW-G8p-antiPD1(LC)-1Pe)

   Plasmid

   #105195

   Purpose

   Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab light chain transcripts containing miR1-Bs(Pe)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   G8p-antiPD1(LC)-1Pe
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Donor-PIGAmut

   Plasmid

   #153548

   Purpose

   Used as a donor vector for the targeted knock-in of a mutation in PIGA exon 6

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
   

   Use

   Donor plasmid

   Tags

   N/A

   Expression

   Mutation

   a 3-bp mutation in exon 6

   Promoter

   No promoter

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Y365A or A365Y inducible rescue

   Plasmid

   #131323

   Purpose

   gRNA to generate i-WT-r or i-MT-r systems

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   Y365A or A365Y inducible rescue
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTF2_KO_ex11_gRNA-4

   Plasmid

   #131334

   Purpose

   gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   MTF2_KO_ex11_gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LMX1B-gRNA-N-del

   Plasmid

   #131333

   Purpose

   gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   LMX1B-gRNA-N-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. HoxD del ds1

   Plasmid

   #131338

   Purpose

   gRNA to delete a nucleation site near HoxD. Use with HoxD del us1

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EVX2-gRNA-C-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. SKOR1-gRNA-C-del

   Plasmid

   #131332

   Purpose

   gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-N-del

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   SKOR1-gRNA-C-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. SKOR1-gRNA-N-del

    Plasmid

    #131331

    Purpose

    gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-C-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    SKOR1-gRNA-N-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. HoxD del control ds

    Plasmid

    #131336

    Purpose

    gRNA to delete control region near HoxD. Use with HoxD del control us

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    CTRL-gRNA-C-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

12. HoxD del us1

    Plasmid

    #131337

    Purpose

    gRNA to delete a nucleation site near HoxD. Use with HoxD del ds1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EVX2-gRNA-N-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. HoxD del control us

    Plasmid

    #131335

    Purpose

    gRNA to delete control region near HoxD. Use with HoxD del control ds

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    CTRL-gRNA-N-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. EED-KO-gRNA-1

    Plasmid

    #131321

    Purpose

    gRNA to knockout EED. Use with EED-KO-gRNA-2

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EED-KO-gRNA-1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

15. pZ_lacO-cP-AOX1-LovC

    Plasmid

    #126729

    Purpose

    yeast plasmid overexpressing LovC

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    lovC
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZ_lacO-cP-AOX1-LovB

    Plasmid

    #126728

    Purpose

    yeast plasmid overexpressing LovB

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    lovB
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    T7227A,T8994A

    Promoter

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. gh96

    Plasmid

    #106756

    Purpose

    expression of gRNA targeting POFUT1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    POFUT1 (POFUT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.2 mir-1-1 reporter mir129-2-2

    Plasmid

    #46728

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir129-2-2 (MIR129-2 Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. CoV2-Spike-D614G-Alpha

    Plasmid

    #177961

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    del69, 70, del144, D614G, N501Y, A570D, P681H, T7…

    Promoter

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-PE2max-BSD

    Plasmid

    #191102

    Purpose

    Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    PEmax-P2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed R221K, N394K, H840A from SpCas9

    Promoter

    EF-1a

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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