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We narrowed to 4,562 results for: TIL

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Showing: 3281 - 3290 of 4562 results

  2. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100902-plasmid-map-sequence-id-195323

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  8. pCAG-hPPP3CB-FLAG

    Plasmid
    #179135
    Purpose
    Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179135-plasmid-map-sequence-id-352807

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Showing: 3281 - 3290 of 4562 results