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We narrowed to 26,120 results for: expression vector

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Showing: 6561 - 6580 of 26120 results

  1. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100902-plasmid-map-sequence-id-195323

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  2. FZ-NbPDS

    Plasmid
    #184268
    Purpose
    pAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.
    Depositor
    Feng Qu
    Article
    Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do
    Insert
    CPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
    Expression
    Plant
    Mutation
    The protease processing site between MP and L-CP …
    Promoter
    CaMV 35S with duplicated enhancer
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184268-plasmid-map-sequence-id-363057

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  7. pUAST-HA, Pbl-A

    Plasmid
    #69792
    Purpose
    Pebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
    Depositor
    Arno Muller
    Article
    van Impel et al Development. 2009 Mar;136(5):813-22. doi
    Insert
    Pebble - Drosophila guanine nucleotide exchange factor
    Use
    P element-based puast vector for gal4-regulated e…
    Tags
    HA tag
    Expression
    Insect
    Mutation
    Encodes Pbl (NP_729306.1) lacking last 9 amino ac…
    Promoter
    hsp70 promoter
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pUAST-HA, Pbl-A DH domain

    Plasmid
    #69794
    Purpose
    Pebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
    Depositor
    Arno Muller
    Article
    van Impel et al Development. 2009 Mar;136(5):813-22. doi
    Insert
    Pebble DH domain
    Use
    P element-based puast vector for gal4-regulated e…
    Tags
    HA-Tag
    Expression
    Insect
    Mutation
    Contains only the Pebble DH domain
    Promoter
    hsp70
    Available Since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pcDNA5-FRT-TO-Nsp13-HF_GC3opt

    Plasmid
    #157716
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157716-plasmid-map-sequence-id-307912

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  13. pcDNA5-FRT-TO-FH-Nsp13_GC3opt

    Plasmid
    #157715
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    FH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157715-plasmid-map-sequence-id-307906

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  15. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

    Plasmid
    #157699
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157699-plasmid-map-sequence-id-307863

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  16. pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt

    Plasmid
    #157692
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    FH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157692-plasmid-map-sequence-id-307834

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  17. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

    Plasmid
    #157693
    Purpose
    mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157693-plasmid-map-sequence-id-307835

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Showing: 6561 - 6580 of 26120 results