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Showing: 6561 - 6580 of 18117 results

  1. pBK-Ma pylRS nitroY/haloY-F5

    Plasmid
    #212125
    Purpose
    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.
    Depositor
    Ryan Mehl
    Insert
    M. alvus PylRS 3-NY/HaloY F5
    Expression
    Bacterial
    Mutation
    CTG125CTT, N166A V168S, A223C, W239R
    Promoter
    GlnS (constitutive)
    Available Since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    212125-plasmid-map-sequence-id-443626

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  4. pJAT21

    Plasmid
    #204309
    Purpose
    For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-GFPn
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204309-plasmid-map-sequence-id-405852

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  5. p663-UBC-miniTurbo-V5-NEK10_IDG-K

    Plasmid
    #189874
    Purpose
    Gateway destination clone of NEK10 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK10 (NEK10 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    189874-plasmid-map-sequence-id-381797

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  6. p663-UBC-miniTurbo-V5-CDK2_IDG-K

    Plasmid
    #158193
    Purpose
    Gateway destination clone of CDK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK2 (CDK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    158193-plasmid-map-sequence-id-373670

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  7. p663-UBC-miniTurbo-V5-CAMKK2_IDG-K

    Plasmid
    #170577
    Purpose
    Gateway destination clone of CAMKK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CAMKK2 (CAMKK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    170577-plasmid-map-sequence-id-372360

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  9. pcDNA5-FRT-TO-hPKAalpha-Flag

    Plasmid
    #185380
    Purpose
    For mammalian expression of human PKAalpha-FLAG
    Depositor
    Ayaz Najafov
    Insert
    PRKACA (PRKACA Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185380-plasmid-map-sequence-id-363613

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  10. PRDX6A-c001

    Plasmid
    #175474
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    PRDX6 (PRDX6 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    See Depositor Comments
    Available Since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    175474-plasmid-map-sequence-id-353175

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  11. CD44Z-c011

    Plasmid
    #175455
    Purpose
    Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    His6
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175455-plasmid-map-sequence-id-353216

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  16. p667-UBC-BCKDK-V5-miniTurbo_IDG-K

    Plasmid
    #158189
    Purpose
    Gateway destination clone of BCKDK (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    BCKDK-V5-miniTurbo (BCKDK Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    158189-plasmid-map-sequence-id-328918

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  17. p663-UBC-miniTurbo-V5-CDK17_IDG-K

    Plasmid
    #158174
    Purpose
    Gateway destination clone of CDK17 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK17 (CDK17 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    158174-plasmid-map-sequence-id-309978

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  18. TBXTA-c027

    Plasmid
    #139764
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, G177D (TBXT Human)
    Tags
    Avi-tag (Biotin) and His6-GST-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids S2- M435
    Promoter
    T7
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139764-plasmid-map-sequence-id-296577

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  19. pHAGE-CMV-Flag-TLK2_IDG-K

    Plasmid
    #135278
    Purpose
    Gateway destination clone of TLK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-TLK2 (TLK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    135278-plasmid-map-sequence-id-267204

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Showing: 6561 - 6580 of 18117 results