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pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)

Plasmid

#159886

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-1(no_atg)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)

Plasmid

#159887

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-2 (no_start)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCZGY2840

Plasmid

#87764

Purpose

Pmec-4-PH::miniSOG, expression of membrane targeted PH-miniSOG in mechanosensory neurons

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmec-4-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pmec-4

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGM59

Plasmid

#74785

Purpose

Expresses 3XFLAG::GR LBD::DAF-16A from the hsp16.48 promoter

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Insert

3X FLAG::GR LBD::DAF-16A (daf-16, NR3C1 Human, Synthetic, Nematode)

Expression

Worm

Promoter

hsp16.48

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pOX + kqt1

Plasmid

#16136

Depositor

Larry Salkoff

Article

Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

Insert

Caenorhabditis elegans potassium channel (kqt-1 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pJW1241

Plasmid

#74904

Purpose

Expresses 3XFLAG::GR LBD::eGFP from the hsp16.48 promoter

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Insert

3X FLAG::GR LBD::eGFP (NR3C1 Human, Synthetic, Nematode)

Expression

Worm

Promoter

hsp16.48

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::GCaMP6s

Plasmid

#124351

Purpose

Nuclear Localized Pan-Neuronal GCaMP6s

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

rab-3, GCaMP6s (rab-3 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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KG#59

Plasmid

#110929

Purpose

Expresses proteins pan-neuronally in C. elegans (entire nervous system)

Depositor

Kenneth Miller

Article

Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

Inserts

rab-3 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEY79

Plasmid

#191074

Purpose

flp-12 fluorescent neural reporter driving nuclear T-Sapphire expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-12 (flp-12 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY62

Plasmid

#191020

Purpose

acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-5 (acr-5 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSDH57

Plasmid

#139047

Purpose

MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mScarlet (no STOP codon)

Expression

Worm

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCZGY2839

Plasmid

#87765

Purpose

Prgef-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in neurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Prgef-1-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Prgef-1

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-DRE-1-pcDNA3.1-

Plasmid

#52513

Purpose

expresses C. elegans DRE-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ2240 - [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159855

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGC630

Plasmid

#107013

Purpose

GFP reporter of lag-2 driven by 2 kb promoter

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Expression

Worm

Promoter

lag-2

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pOX + Shaker1a

Plasmid

#16139

Depositor

Larry Salkoff

Article

Fawcett et al J Biol Chem. 2006 Oct 13. 281(41):30725-

Insert

Voltage-gated K+ channels (ZK1321.2 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pCFJ326 - operon for germline expression

Plasmid

#34875

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

tbb-2 gld-1 operon

Use

Worm targeting

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pSAH85 - [AWCp | wrmScarlet | tbb-2 UTR]

Plasmid

#200359

Purpose

mScarlet BioPart for AWC neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

Depositor

Christian Froekjaer-Jensen

Insert

[AWCp | wrmScarlet | tbb-2 UTR]

Expression

Worm

Promoter

WBGene00012770, srt-47

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCM5.48

Plasmid

#19385

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

mex-5 3'utr in pDONRP2RP3 (mex-5 Nematode)

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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L4440-daf-16af

Plasmid

#31506

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16a/f common (daf-16 Nematode)

Use

RNAi

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only