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Showing: 961 - 990 of 1018 results

  3. SiC-V2-Cas9G7

    Plasmid
    #133043
    Purpose
    Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.
    Depositor
    Sriram Neelamegham
    Article
    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1
    Insert
    Tet repressor
    Use
    Lentiviral
    Tags
    Cerulean
    Expression
    Mutation
    Promoter
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    133043-plasmid-map-sequence-id-264424

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  5. SSTR1-Tango

    Plasmid
    #66502
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    SSTR1 (SSTR1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. RBD-Fc

    Plasmid
    #164221
    Purpose
    Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter
    Depositor
    Sriram Neelamegham
    Article
    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1
    Insert
    Spike RBD (S SARS-CoV)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    164221-plasmid-map-sequence-id-323637

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  11. SSTR1-DuET

    Plasmid
    #213376
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR1 (SSTR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213376-plasmid-map-sequence-id-430732

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  13. pCS-CG-WT-Cer-VWF

    Plasmid
    #124827
    Purpose
    Expresses multimeric human VWF-ECFP fusion protein. The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.
    Depositor
    Sriram Neelamegham
    Article
    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:
    Insert
    VWF with cyan fluorescent protein fusion between D'D3 and A1 domains (VWF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124827-plasmid-map-sequence-id-243867

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  23. ACE2

    Plasmid
    #164219
    Purpose
    Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker
    Depositor
    Sriram Neelamegham
    Article
    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1
    Insert
    ACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    silent mutation to remove internal EcoRI site (GA…
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    164219-plasmid-map-sequence-id-323632

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  29. pCS-CG-Lock-Cer-VWF

    Plasmid
    #124828
    Purpose
    Expresses multimeric human VWF-ECFP fusion protein with A2-Lock domain The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.
    Depositor
    Sriram Neelamegham
    Article
    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:
    Insert
    VWF with cyan fluorescent protein fusion between D'D3 and A1 domains and Lock A2 domain (VWF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124828-plasmid-map-sequence-id-243927

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Showing: 961 - 990 of 1018 results