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We narrowed to 9,448 results for: Coli

Showing: 1601 - 1650 of 9448 results
  1. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Available Since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
  2. pBDC

    Plasmid
    #135188
    Purpose
    Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.
    Depositor
    Insert
    CmR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    None
    Promoter
    cat
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pBDK

    Plasmid
    #135190
    Purpose
    Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection
    Depositor
    Insert
    KanR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    cat
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pASKt15C+SrtA7

    Plasmid
    #64986
    Purpose
    Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Insert
    sortase A
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, E105K, E108A, D16…
    Promoter
    tetracycline promoter/operator
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pHL 1405

    Plasmid
    #53010
    Purpose
    pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp
    Depositor
    Inserts
    RhyB sRNA
    DsrA sRNA
    hfq
    sodB
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains -56 to +141 relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pHL 100

    Plasmid
    #52989
    Purpose
    pLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfp
    Depositor
    Inserts
    OxyS sRNA
    fhlA
    Use
    Synthetic Biology
    Tags
    GFP and RBS-mCherry
    Expression
    Bacterial
    Mutation
    −309 to +96 bp relative to start codon
    Promoter
    pLlac0-1 and pLtetO-1
    Available Since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. pKL001

    Plasmid
    #98917
    Purpose
    Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.
    Depositor
    Insert
    CaPR
    Expression
    Bacterial
    Promoter
    BBa_J23118 - Strong Constitutive
    Available Since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pSS9

    Plasmid
    #71655
    Purpose
    Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coli
    Depositor
    Insert
    gfpuv
    Use
    Synthetic Biology
    Promoter
    T7A1
    Available Since
    Jan. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
  9. pTJV1Sc-rpoB

    Plasmid
    #166978
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Inserts
    Ec86 Retron (ECD_00831 )
    lambda bet
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23101 and VanCC
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pMAL_dstβL

    Plasmid
    #73805
    Purpose
    Plasmid for selection for insertion and homodimerization
    Depositor
    Inserts
    CAT Marker
    ToxR
    beta lactamase
    spectomycin resistance
    CLS TM domain
    Tags
    Part Of the dstbl chiemra, Part of the dsTbL chim…
    Expression
    Bacterial
    Promoter
    NaN and ToxR promotor
    Available Since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
  11. pHL 1075

    Plasmid
    #53004
    Purpose
    pCon:fhlA::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Inserts
    fhlA
    sodB
    Use
    Synthetic Biology
    Tags
    GFP and mCherry
    Expression
    Bacterial
    Mutation
    contains −309 to +96 bp relative to start codon a…
    Promoter
    pCon and pLlacO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  12. pANY6

    Plasmid
    #112198
    Purpose
    This plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.
    Depositor
    Type
    Empty backbone
    Tags
    ELP-tag
    Expression
    Bacterial
    Available Since
    Aug. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pASKt15C+SrtA5

    Plasmid
    #64985
    Purpose
    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Insert
    sortase A
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, D160N, D165A, K19…
    Promoter
    tetracycline promoter/operator
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1601 - 1650 of 9448 results