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Plasmid#45142DepositorInsertE-coli-pathways-QconCAT14
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET29b_Qcat11
Plasmid#45139DepositorInsertE-coli-pathways-QconCAT11
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET29b_Qcat8
Plasmid#45136DepositorInsertE-coli-pathways-QconCAT8
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET29b_Qcat4
Plasmid#45132DepositorInsertE-coli-pathways-QconCAT4
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET29b_Qcat17
Plasmid#45145DepositorInsertE-coli-pathways-QconCAT17
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pANY3
Plasmid#112197PurposeThis plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.DepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHL 1405
Plasmid#53010PurposepLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA
hfq
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains -56 to +141 relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACCS3-1
Plasmid#78559Purpose4.7 kb HindIII ETEC CS3 fimbrial operon from pCS100 cloned into pACYC184.DepositorInsertcs3 operon
UseTagsExpressionBacterialMutationPromotertetAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL1855
Plasmid#53041PurposePLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsLVA, RBS(st3), and glgC leaderExpressionBacterialMutationPromoterpConNoHind and pLlac0-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACYC-yhaM
Plasmid#207650PurposeOverexpression of native L-cysteine desulfidase in E. coli MG1655DepositorInsertyhaM
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-decR
Plasmid#207653PurposeOverexpression of L-cysteine desulfidase transcriptional regulator in E. coli MG1655DepositorInsertdecR
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMF2-yhaO
Plasmid#207654PurposeOverexpression of L-cysteine importer in E. coli MG1655DepositorInsertyhaO
UseTags6xHisExpressionBacterialMutationPromoteraraBpAvailable sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaTW-R1
Plasmid#210455PurposeExpress waaT and waaW from E. coli R1 under the control of PBADDepositorInsertwaaT and waaW
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaTW-R4
Plasmid#210456PurposeExpress waaT and waaW from E. coli R4 under the control of PBADDepositorInsertwaaT and waaW
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXU01
Plasmid#212151PurposePlasmid expressing xylitol dehydrogenase under inducible promoter PT7 control. Plasmid confers the ability to use xylitol as major carbon source to E. coli.DepositorInsertxylitol dehydrogenase
UseTags6x HisExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ4-PdegV-LacZ
Plasmid#191620PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), PdegV promoter-lacZ, E. lenta constitutive promoterDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ8-CadR-Pcadh-LacZ
Plasmid#191623PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadRDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ8.1-Pcadh-LacZ
Plasmid#191624PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ9-Phcdh-LacZ
Plasmid#191625PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTE1069
Plasmid#186629PurposeSCB-GFP E. coli reporter plasmid. Encodes repressor ScbR and ScbAp promoter upstream of GFP.DepositorInsertsscbR
scbAp promoter
UseSynthetic BiologyTags6xArgExpressionBacterialMutationPromoterBBa_J23100 and scbApAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-pntAB
Plasmid#158609PurposeLow phosphate induction of pntABDepositorInsertpntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)
UseTagsExpressionBacterialMutationPromoterInsulated ugpBp from E. coliAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
AraC-pHG165c WT
Plasmid#170114PurposeConstitutively expressed, wild-type E coli AraC transcription activator; activated by L-Arabinose.DepositorInsertAraC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
EcNusGNTD
Plasmid#113123PurposeExpresses N-terminus of E. coli NusGDepositorInsertNusG
UseTagsHis6-MBP-N10-TEVExpressionBacterialMutationDeleted residues 120-181PromoterT7Available sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-TTM
Plasmid#127794PurposeExpresses the TTM/CYTH domain of ygiF in E. coli cellsDepositorInsertygiF (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 1-200)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_W22HDHFR_WTTS
Plasmid#91241Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationW22HPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31VDHFR_WTTS
Plasmid#91227Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHL 100
Plasmid#52989PurposepLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfpDepositorInsertsOxyS sRNA
fhlA
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutation−309 to +96 bp relative to start codonPromoterpLlac0-1 and pLtetO-1Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1853
Plasmid#53040PurposePLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsLVA, RBS(st2), RBS(st3), and glgC leaderExpressionBacterialMutationPromoterpConNoHind and pLlac0-1Available sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 990
Plasmid#52992PurposepLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfpDepositorInsertsDsrA sRNA
RhyB sRNA (competitor)
rpoS
UseSynthetic BiologyTagsgfpExpressionBacterialMutationincludes −150 to +30 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1022
Plasmid#52996PurposepLtetO-1:RhyB, pCon:OxyS, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
OxyS sRNA
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 802
Plasmid#52991PurposepLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfpDepositorInsertsOxyS sRNA
ompC
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutationcontains −81 to +36 bp relative to start codonPromoterpLlacO-1 and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1020
Plasmid#52995PurposepLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA (competitor)
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1019
Plasmid#52994PurposepLtetO-1:RhyB, pCon:MicC, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
MicC sRNA (Competitor)
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1039
Plasmid#53000PurposepLtetO-1:DsrA, pCon:OxyS, pLlacO-1:rpoS::gfpDepositorInsertsDsrA sRNA
OxyS sRNA (competitor)
rpoS
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −150 to +30 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1073
Plasmid#53003PurposepCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsmCherry
GFP
UseSynthetic BiologyTagsompC (target mRNA) and sodB (target mRNA)ExpressionBacterialMutationcontains −56 to +141 bp relative to start codon a…PromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1303
Plasmid#53008PurposepLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfpDepositorInsertssodB
ompC
UseSynthetic BiologyTagsGFP and mCherryExpressionBacterialMutationcontains -56 to +141 relative to start codon and …PromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GFP TEV LIC cloning vector (1GFP)
Plasmid#29663DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, GFP, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV LIC cloning vector (1M)
Plasmid#29656DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, MBP, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV LIC cloning vector (2M-T)
Plasmid#29708DepositorTypeEmpty backboneUseTags6xHis, MBP, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV LIC cloning vector (1S)
Plasmid#29659DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, SUMO, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV LIC cloning vector (2S-T)
Plasmid#29711DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, SUMO, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pExp-TF
Plasmid#112575PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-TriggerFactor and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-MsyB
Plasmid#112570PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8His and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)
Plasmid#29722DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pExp-DsbC
Plasmid#112564PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-DsbC and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GST TEV LIC cloning vector (2G-T)
Plasmid#29707DepositorTypeEmpty backboneUseTags6xHis, GST, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_PgyrA_gfp
Plasmid#212138PurposeCargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)
Plasmid#55209DepositorTypeEmpty backboneUseTags10xAsn, 10xHis, MPB, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
bU6-sgNeo1-mU6-sgNT-hU6-sgNeo2
Plasmid#177231PurposeExpresses neomycin gRNA's ( bU6 and hU6 ), non-targeting gRNA ( mU6 ) and Cre-recombinaseDepositorInsertsgNeo1/sgNT1/sgNeo2
UseLentiviralTagsExpressionMutationPromoterbU6/mU6/hU6Available sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExp-Trx
Plasmid#112577PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-TrxA and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only