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pCMVmyc-ORF73SOCS

Plasmid

#21546

Depositor

J Simas

Article

Rodrigues et al EMBO J. 2009 May 6. 28(9):1283-95.

Insert

ORF73 (ORF73 MuHV-4)

Tags

Myc

Expression

Mammalian

Mutation

amino acids at positions 199, 202, 203 and 206 we…

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
2C-EGFP

Plasmid

#69071

Purpose

Expresses EGFP under MERVL_LTR

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

EGFP

Expression

Mammalian

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Article

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGagMBPHis

Plasmid

#42176

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

Gag protein sequence is from HIV-1 clone NL4-3

Tags

6xHIS and MBP

Expression

Bacterial

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only
Beta-arrestin2 GFP WT

Plasmid

#35411

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

Insert

βarrestin2 (Arrb2 Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pRS405-FlagPEN2/PS1wt

Plasmid

#207803

Purpose

Yeast integrative vector for expression of FlagPEN2 and Presenilin 1

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits PEN-2 and presenilin1 wt

Expression

Yeast

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pRS402-nicastrin/APH-1a-HA

Plasmid

#207804

Purpose

Yeast integrative vector for expression of Nicastrin and APH-1a-HA

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits nicastrin and Aph1a

Expression

Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMSP1E1D1

Plasmid

#161605

Purpose

Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E1D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcD)

Plasmid

#176191

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Tags

6xHis

Expression

Bacterial

Promoter

Ptac

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMSP1E2D1

Plasmid

#161606

Purpose

Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E2D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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InterCatch-3xL

Plasmid

#186909

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)3 linker and the other side expressing EGFP and puromycin resistance gene

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

TfR-(GGGGS)3-SpyCatcher003-sfCherry1-10

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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InterTag-3xL

Plasmid

#186913

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side expressing BFP and puromycin resistance gene

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-(GGGGS)3-PDGFR

Expression

Mammalian

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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BASIC_8_RBS34- mCherry -B0015

Plasmid

#68142

Purpose

storage vector for BASIC part: RBS-RFP(ORF)

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

RBS34-mcherry-B0015

Expression

Bacterial

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-HIRA-polyA

Plasmid

#59781

Purpose

Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCBFRE-(mt)-luc

Plasmid

#26896

Depositor

Nicholas Gaiano

Article

Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

Insert

CBFRE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated CBF1 sites (see note)

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
YFP Gamma 9

Plasmid

#36107

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 9

Tags

YFP

Expression

Mammalian

Mutation

HIs tag between YFP and insert

Promoter

CMV

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
BASIC_11_RBS34-GFP-B0015

Plasmid

#68145

Purpose

storage vector for BASIC part: RBS-GFP(ORF)

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

RBS-34-GFP-B0015

Expression

Bacterial

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pZS220

Plasmid

#177642

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Mutation

K47A

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-RCC1-polyA

Plasmid

#59762

Purpose

Express Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

RCC1

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pUAS-MEYFP

Plasmid

#86372

Purpose

membrane targeted EYFP expression in zebrafish

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

EYFP

Use

Zebrafish

Tags

GAP43 membrane targeting

Promoter

UAS

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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