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YCe3159 HC_Kan_conn_W-X

Plasmid

#100666

Purpose

EMMA toolkit Assembly connector W-X

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector W-X

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2066 HC_Kan_conn_Y-Z

Plasmid

#100667

Purpose

EMMA toolkit Assembly connector Y-Z

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector Y-Z

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe3731 HC_Kan_conn_A-B

Plasmid

#100638

Purpose

EMMA toolkit Assembly connector A-B

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector A-B

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe3732 HC_Kan_conn_B-C

Plasmid

#100639

Purpose

EMMA toolkit Assembly connector B-C

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector B-C

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1915 HC_Kan_conn_A-C

Plasmid

#100640

Purpose

EMMA toolkit Assembly connector A-C

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector A-C

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2024 HC_Kan_conn_D-E

Plasmid

#100641

Purpose

EMMA toolkit Assembly connector D-E

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector D-E

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe3756 HC_Kan_conn_E-F

Plasmid

#100642

Purpose

EMMA toolkit Assembly connector E-F

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector E-F

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
YCe1917 HC_Kan_conn_D-F

Plasmid

#100643

Purpose

EMMA toolkit Assembly connector D-F

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector D-F

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCMVmyc-ORF73SOCS

Plasmid

#21546

Depositor

J Simas

Article

Rodrigues et al EMBO J. 2009 May 6. 28(9):1283-95.

Insert

ORF73 (ORF73 MuHV-4)

Tags

Myc

Expression

Mammalian

Mutation

amino acids at positions 199, 202, 203 and 206 we…

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Article

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMSP1E1D1

Plasmid

#161605

Purpose

Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E1D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pH2B-mPlum/Gag

Plasmid

#102255

Purpose

co-expression of Gag and H2B Plum in mammalian cells

Depositor

Suliana Manley

Article

Gunzenhauser et al PLoS One. 2014 Dec 10;9(12):e115095. doi

Inserts

Gag
H2B Plum

Expression

Mammalian

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Beta-arrestin2 GFP WT

Plasmid

#35411

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

Insert

βarrestin2 (Arrb2 Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pMSP1E2D1

Plasmid

#161606

Purpose

Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E2D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 9

Plasmid

#36107

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 9

Tags

YFP

Expression

Mammalian

Mutation

HIs tag between YFP and insert

Promoter

CMV

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pRS405-FlagPEN2/PS1wt

Plasmid

#207803

Purpose

Yeast integrative vector for expression of FlagPEN2 and Presenilin 1

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits PEN-2 and presenilin1 wt

Expression

Yeast

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pRS402-nicastrin/APH-1a-HA

Plasmid

#207804

Purpose

Yeast integrative vector for expression of Nicastrin and APH-1a-HA

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits nicastrin and Aph1a

Expression

Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGagMBPHis

Plasmid

#42176

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

Gag protein sequence is from HIV-1 clone NL4-3

Tags

6xHIS and MBP

Expression

Bacterial

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-HIRA-polyA

Plasmid

#59781

Purpose

Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCBFRE-(mt)-luc

Plasmid

#26896

Depositor

Nicholas Gaiano

Article

Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

Insert

CBFRE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated CBF1 sites (see note)

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only