We narrowed to 1,412 results for: Flp

1. pNM3734

   Plasmid

   #154118

   Purpose

   mec-4 promoter driving GAL4-QFAD in an RCME vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   mec-4p::Gal4SK::QFAD::tbb-2 '3
   

   Use

   Rmce

   Expression

   Worm

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNM3649

   Plasmid

   #154110

   Purpose

   miniMos dual component RMCE landing site vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   loxP::SEC::loxP::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
   

   Use

   Minimos

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDOCKING-Neo

   Plasmid

   #31441

   Depositor

   Gerhart Ryffel

   Article

   Waldner et al BMC Res Notes. 2011 Oct 18;4:420.

   Insert

   ECFP-neomycin
   

   Use

   Cre/Lox; Phic31/attp

   Tags

   ECFP

   Expression

   Mammalian

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIJ8

   Plasmid

   #68122

   Purpose

   Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

   Depositor

   Alex Nielsen

   Article

   Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

   Inserts

   flp recombinase
   rhaS
   rhaR
   

   Expression

   Bacterial

   Promoter

   rhamnose

   Available Since

   Dec. 8, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pFUdioFRTeYFPW

   Plasmid

   #133998

   Purpose

   expresses eYFP upon coexpression with the recombinase flippase (Flp)

   Depositor

   Elly Nedivi

   Article

   Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

   Insert

   eYFP
   

   Use

   Lentiviral; Flp/frt

   Expression

   Mammalian

   Mutation

   inverted and flanked by two incompatible FRT site…

   Promoter

   UbC

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2-eab2-[GTR]

   Plasmid

   #46144

   Depositor

   Wenbiao Chen

   Article

   Boniface et al Genesis. 2009 Jul;47(7):484-91. doi: 10.

   Insert

   EGFP/mCherry
   

   Use

   Cre/Lox; Tol2 zebrafish transgenic expression

   Available Since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNM3749

   Plasmid

   #154108

   Purpose

   loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Inserts

   LoxP-SEC-LoxP
   FRT::rpl-28p::GFP::his-58::FRT3
   

   Use

   Cre/Lox; Rmce

   Expression

   Worm

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA

   Plasmid

   #154867

   Purpose

   Flp-dependent hM4D(Gi) DREADD-mCherry

   Depositor

   Ulrik Gether

   Article

   Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

   Has Service

   AAV Retrograde and AAV8

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV; Flp-frt

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   Human Synapsin-1

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

   Plasmid

   #105802

   Purpose

   Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: 3C-mRuby3; ORF2: TEV-mClover3

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid

    #105801

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: FLAG-3C; ORF2: mClover3-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

    Plasmid

    #105805

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Venus

    Expression

    Mammalian

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

    Plasmid

    #105803

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-FLAG; ORF2: TEV-mClover3

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid

    #105806

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Amber

    Expression

    Mammalian

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. tubP-FRT>GAL80-6-FRT>

    Plasmid

    #63173

    Purpose

    ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "Flp-Out" GAL80
    

    Expression

    Insect

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pADH99

    Plasmid

    #90979

    Purpose

    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    US_HIS1
    FRT
    pENO1
    C. albicans Cas9
    pMAL2
    FLP
    NAT 1 of 2
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKF-P14MM2AG5h

    Plasmid

    #128254

    Purpose

    Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    rtTA-Advanced::P2A::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDN-D2irTNG5kwh

    Plasmid

    #128279

    Purpose

    Mammalian linearizer gene circuit in a Flp-In expression system with a hTetR::EGFP fusion protein

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    hTetR::NLS::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV-D2ir promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3

    Plasmid

    #67276

    Purpose

    RMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PA

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

    Inserts

    GFP (mWasabi)
    hygro
    

    Tags

    F3 and Frt

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDN-D2irTN2AG5kwh

    Plasmid

    #128253

    Purpose

    Mammalian linearizer gene circuit in a Flp-In expression system based on negative feedback. The hTetR and EGFP is separated by a P2A motif.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    Beta Globin Intron::hTetR::P2A::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Expression

    Mammalian

    Promoter

    CMV D2ir promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKF-D2irTNP2AG-T2APuroR-5kwh

    Plasmid

    #128255

    Purpose

    Mammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    hTetR::P2A::EGFP::T2A::PuroR
    

    Use

    Synthetic Biology; Flp-in expression vector

    Expression

    Mammalian

    Promoter

    CMV-D2ir promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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