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HBR-6U

Plasmid

#42621

Depositor

Hannele RuoholaBaker

Article

Zhou et al PLoS One. 2011;6(11):e27460. doi: 10.137

Insert

6 x HIF binding element

Use

Lentiviral

Tags

eYFP

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagD 121L

Plasmid

#19309

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Tags

GST and HA

Expression

Mammalian

Mutation

Q121L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pSC201plus

Plasmid

#164227

Purpose

Integrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression

Depositor

Silvia Cardona

Article

Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

Rhamnose-inducible

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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FRP792_insul-(lexA-box)2-PminCYC1-Citrine-TCYC1

Plasmid

#58433

Purpose

Induces Citrine expression from a promoter containing 2 lexA boxes in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)2-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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FRP791_insul-(lexA-box)1-PminCYC1-Citrine-TCYC1

Plasmid

#58432

Purpose

Induces Citrine expression from a promoter containing 1 lexA box in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)1-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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FRP800_insul-(lexA-box)3-PminCYC1-Citrine-TCYC1

Plasmid

#58436

Purpose

Induces Citrine expression from a promoter containing 3 lexA boxes in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)3-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y819W

Plasmid

#1307

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y819W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 819 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 L892W

Plasmid

#1308

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L892W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 892 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 F130W

Plasmid

#1302

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 F130W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Phe 130 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 L553W

Plasmid

#1306

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L553W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 553 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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2M-T-Mce3R_K262A

Plasmid

#225728

Purpose

To express Mce3R mutant K262A in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Mutation

K262A

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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2M-T_Mce3R

Plasmid

#225726

Purpose

To express Mce3R in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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2M-T-Mce3R_R53A

Plasmid

#225727

Purpose

To express Mce3R mutant R53A in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R53A

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-LASV-GPC-HA-c3xFLAG

Plasmid

#215691

Purpose

Produces the Lassa virus glycoprotein with a c-terminal HA tag and 3xFLAG tag

Depositor

Melinda Brindley

Article

Willard et al Pathogens. 2018 Dec 26;8(1):1. doi: 10.3

Insert

LASV GP-HA-FLAG

Tags

HA-3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSuperior.retro.puro-sh-γ-tubulin

Plasmid

#205602

Purpose

Tet-inducible expression of shRNA targeting gamma-tubulin in human cells, retroviral

Depositor

Tarun Kapoor

Article

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

sh-g-Tubulin

Expression

Mammalian

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y257W

Plasmid

#1303

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y257W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 257 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y507W

Plasmid

#1305

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y507W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 507 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104b F130W

Plasmid

#1309

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 F130W (HSP104 Budding Yeast)

Expression

Yeast

Mutation

Changed Phe 130 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104b Y257W

Plasmid

#1310

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y257W (HSP104 Budding Yeast)

Expression

Yeast

Mutation

Changed Tyr 257 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104b Y466W

Plasmid

#1311

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y466W (HSP104 Budding Yeast)

Expression

Yeast

Mutation

Changed Tyr 466 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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