We narrowed to 870 results for: Px330

1. C-6-BE

   Plasmid

   #135034

   Purpose

   C-T base editing using LjDA1L2_2 fused to C terminal of nCas9

   Depositor

   Zilong Qiu, Tian-Lin Cheng

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   LjDA1L2_2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 with EF-1alpha intron

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. C-11-BE

   Plasmid

   #135039

   Purpose

   C-T base editing using LpCDA1L2_2 fused to C terminal of nCas9

   Depositor

   Zilong Qiu, Tian-Lin Cheng

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   LpCDA1L2_2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 with EF-1alpha intron

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLHDR_R1

   Plasmid

   #124209

   Purpose

   gRNA/Cas9 expression for 2nd editing using pSLHDR01, 02

   Depositor

   Matthew Porteus

   Article

   Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

   Insert

   gRNA
   

   Use

   CRISPR

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 7i sgRNA for 4-μHOM reporter

   Plasmid

   #113626

   Purpose

   sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7i sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

5. 7b sgRNA for EJ7-GFP reporter

   Plasmid

   #113624

   Purpose

   sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7b sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

6. 7h sgRNA for 4-μHOM reporter

   Plasmid

   #113625

   Purpose

   sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7h sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

7. ER50-SpCas9-ER50

   Plasmid

   #85448

   Purpose

   Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   ER50-SpCas9-ER50
   

   Use

   AAV

   Tags

   destabilized domain of estrogen receptor ligand b…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pREDIT_Cas9-MS2-BB_BbsI

   Plasmid

   #164802

   Purpose

   REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Halo-TCAB1 KI sgRNA

   Plasmid

   #207611

   Purpose

   sgRNA for HaloTag insertion into the endogenous TCAB1 locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   CCAAAGTCTTCATACTGCAG
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. N-terminal Halo-XLF

    Plasmid

    #207546

    Purpose

    Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    HaloTag with internal PuroR cassette flanked by human Lig4 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. USP19 sgRNA2

    Plasmid

    #78586

    Purpose

    delete USP19 gene in human cell

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 sgRNA2 (USP19 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

12. N-Terminal Halo XRCC4

    Plasmid

    #207541

    Purpose

    Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    HaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. N-terminal Halo-Ku70

    Plasmid

    #207547

    Purpose

    Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    HaloTag with flanked by human Ku70 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. sgRNA TR KO 2

    Plasmid

    #207610

    Purpose

    sgRNA 2 to knockout TR by replacement with a PuroR cassette

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    TCAGGCCGCAGGAAGAGGAA
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. psgRNA-GST-EGFP-GPIBY55

    Plasmid

    #213720

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Promoter

    CMV

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. psgRNA-GST-EGFP-GPIDAF

    Plasmid

    #213719

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Promoter

    CMV

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. psgRNA-GST-EGFP-GPICEAM7

    Plasmid

    #213721

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgRNA TR KO 1

    Plasmid

    #207609

    Purpose

    sgRNA 1 to knockout TR by replacement with a PuroR cassette

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    ACCCTAACTGAGAAGGGCGT
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. DHFR-SpCas9-DHFR

    Plasmid

    #85447

    Purpose

    Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    DHFR-SpCas9-DHFR
    

    Use

    AAV

    Tags

    Destabilized domain (C-terminus version) of E.col…

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. eSpCas9(1.1)_No_FLAG_AAVS1_T2

    Plasmid

    #79888

    Purpose

    Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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