Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 27,977 results for: STI

Clear keywords
Showing: 641 - 660 of 27977 results

  1. pCMV-T7-SpCas9(KKKCMN)-P2A-EGFP (AHK131)

    Plasmid
    #223078
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KKKCMN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KKKCMN)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(KKKCMN)=D1135K, S1136K, G1218K, E1219C, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223078-plasmid-map-sequence-id-447649

    Enlarge

  2. pCMV-T7-SpCas9(ARGIMR)-P2A-EGFP (AHK147)

    Plasmid
    #223069
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(ARGIMR)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(ARGIMR)=D1135A, S1136R, G1218G, E1219I, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223069-plasmid-map-sequence-id-447645

    Enlarge

  3. pCMV-T7-SpCas9(ARTWGR)-P2A-EGFP (RAS2995)

    Plasmid
    #223070
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARTWGR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(ARTWGR)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(ARTWGR)=D1135A, S1136R, G1218T, E1219W, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223070-plasmid-map-sequence-id-447646

    Enlarge

  4. pJK681

    Plasmid
    #72271
    Purpose
    Produces Synechocystis PCC6803 orf sll1489 (synthetic gene)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein SYNGTS_3005
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72271-plasmid-map-sequence-id-131742

    Enlarge

  5. pJK588

    Plasmid
    #72270
    Purpose
    Synthetic gene corresponding to Synechocystis PCC6803 orf sll1489
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein SYNGTS_3005
    Use
    Gene cloning
    Tags
    His6 and TEV cleavage site
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72270-plasmid-map-sequence-id-131740

    Enlarge

  6. pTwist-Kan-MC-L0-TaBSS2-5PU

    Plasmid
    #242009
    Purpose
    L0 Pro+5U Golden Gate part for 5' UTR, core promoter, and upstream regulatory regions of Triticum aestivum TraesCS2B02G399800
    Depositor
    Cristobal Uauy
    Insert
    TaBSS2-5PU
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242009-plasmid-map-sequence-id-504510

    Enlarge

  7. pTwist-Kan-HC-L0-TaBSS1-3D

    Plasmid
    #242008
    Purpose
    L0 3U* Golden Gate part of 3' UTR and downstream regulatory regions of Triticum aestivum MANY-NODED DWARF 1 (MND1) B-subgenome homoeolog (TraesCS7B02G413900)
    Depositor
    Cristobal Uauy
    Insert
    TaBSS1-3D
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242008-plasmid-map-sequence-id-487882

    Enlarge

  8. pUC-GW-Kan-L0-TaSEP1B6(int)

    Plasmid
    #242006
    Purpose
    L0 CDS1 Golden Gate part of the pre-mRNA (excluding UTRs) of Triticum aestivum SEPALLATA1-6 (SEP1-6) B-subgenome homoeolog (TraesCS5B02G396700)
    Depositor
    Cristobal Uauy
    Insert
    TaSEP1-B6_with_introns
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242006-plasmid-map-sequence-id-504502

    Enlarge

  9. pLenti- V6.3 Ultra

    Plasmid
    #106172
    Purpose
    lentiviral CMV driven eGFP-P2A-T2A, Blasticidin selectable
    Depositor
    Ewa Snaar-Jagalska
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    T2A, P2A
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106172-plasmid-map-sequence-id-207443

    Enlarge

  10. pTwist-Kan-HC-L0-TaBSS1-5PU

    Plasmid
    #242007
    Purpose
    L0 Pro+5U Golden Gate part of 5'UTR, core promoter, and upstream regulatory regions of Triticum aestivum MANY-NODED DWARF 1 (MND1) B-subgenome homoeolog (TraesCS7B02G413900)
    Depositor
    Cristobal Uauy
    Insert
    TaBSS1-5PU
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242007-plasmid-map-sequence-id-504528

    Enlarge

  11. pTwist-Kan-HC-L0-TaMOFB1(int)

    Plasmid
    #242005
    Purpose
    L0 CDS1 Golden Gate part of the pre-mRNA (excluding UTRs) of Triticum aestivum MORE FLORET 1 (MOF-1) / MULTI-FLORET SPIKELET 2 (MFS-2) B-subgenome homoeolog (TraesCS2B02G420900)
    Depositor
    Cristobal Uauy
    Insert
    TaMOF-B1_with_introns
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242005-plasmid-map-sequence-id-487883

    Enlarge

  12. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

    Enlarge

  13. pLenti- V6.3 Ultra-Chili

    Plasmid
    #106173
    Purpose
    lentiviral CMV driven tdTomato-P2A-T2A, Blasticidin selectable
    Depositor
    Ewa Snaar-Jagalska
    Insert
    dTomato
    Use
    Lentiviral
    Tags
    T2A, P2A
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106173-plasmid-map-sequence-id-207442

    Enlarge

  14. pPB-CAG-NPHP1

    Plasmid
    #209769
    Purpose
    Human NPHP1 (Nephrocystin 1) gene is inserted into pPB-PGK-destination (Plasmid #60436).
    Depositor
    Yohei Hayashi
    Insert
    NPHP1 (NPHP1 Synthetic, Human)
    Use
    Piggybac transposon
    Tags
    HA tag
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    209769-plasmid-map-sequence-id-417213

    Enlarge

  20. pPSU1

    Plasmid
    #89439
    Purpose
    High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders
    Depositor
    Song Tan
    Article
    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.
    Inserts
    500 bp EcoRV fragment
    1000 bp EcoRV fragment
    1500 bp EcoRV fragment
    2000 bp EcoRV fragment
    500 bp PstI fragment
    700 bp PstI fragment
    800 bp PstI fragment (EHMT2 Human)
    900 bp PstI fragment
    1000 bp PstI fragment (BMI1 Human)
    2000 bp PstI fragment
    Expression
    Bacterial
    Available Since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89439-plasmid-map-sequence-id-190157

    Enlarge

Showing: 641 - 660 of 27977 results