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pBIG1a-PAF1CdeltaWDR61

Plasmid

#231745

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Inserts

CTR9 (CTR9 Human)
CDC73 (CDC73 Human)
PAF1 (PAF1 Human)
LEO1 (LEO1 Human)

Use

Tags

3C-Strep(II) and Flag-3C

Expression

Insect

Mutation

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1a-PAF1CdeltaCDC73

Plasmid

#231746

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Inserts

CTR9 (CTR9 Human)
PAF1 (PAF1 Human)
LEO1 (LEO1 Human)
WDR61 (SKIC8 Human)

Use

Tags

3C-Strep(II)

Expression

Insect

Mutation

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1a-PAF1CdeltaLEO1

Plasmid

#231747

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Inserts

CTR9 (CTR9 Human)
CDC73 (CDC73 Human)
PAF1 (PAF1 Human)
WDR61 (SKIC8 Human)

Use

Tags

3C-Strep(II) and Flag-3C

Expression

Insect

Mutation

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mEGFP-HMGB1-MUT-Patchless

Plasmid

#194553

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "Patchless" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44, truncation by stop codon after Lys209

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-TetON-mEGFP-HMGB1-MUT

Plasmid

#194562

Purpose

Dox-inducible Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-45b-mEGFP-HMGB1-K184Rfs

Plasmid

#194544

Purpose

Bacterial Expression of 6xHis mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Bacterial

Mutation

K184Rfs*44

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mEGFP-HMGB1-MUT-RtoK

Plasmid

#194559

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R > K" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

R > K substitutions after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mEGFP-HMGB1-MUT-KtoR

Plasmid

#194560

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "K > R" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K > R substitutions after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-TetON-mEGFP-HMGB1-MUT-Patchless

Plasmid

#194563

Purpose

Dox-inducible Mammalian Expression of mEGFP-HMGB1 Mutant "Patchless" (K184Rfs*44, "Patchless" variant) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44, truncation by stop codon after Lys209

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mEGFP-HMGB1-MUT-Rdel

Plasmid

#194556

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R del" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Deletion of Arginines after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mEGFP-HMGB1-MUT-RorKtoA

Plasmid

#194558

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R or K > A" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

R or K > A substitutions after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mEGFP-HMGB1-MUT-RtoA

Plasmid

#194557

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R > A" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

R > A substitutions after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1a-PAF1C-CDC73deltaKIM

Plasmid

#231748

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Inserts

CTR9 (CTR9 Human)
CDC73 (CDC73 Human)
PAF1 (PAF1 Human)
LEO1 (LEO1 Human)
WDR61 (SKIC8 Human)

Use

Tags

3C-Strep(II) and Flag-3C

Expression

Insect

Mutation

CDC73(delta290-324)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1a-PAF1C-CDC73-YY/AA

Plasmid

#231749

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Inserts

CTR9 (CTR9 Human)
CDC73 (CDC73 Human)
PAF1 (PAF1 Human)
LEO1 (LEO1 Human)
WDR61 (SKIC8 Human)

Use

Tags

3C-Strep(II) and Flag-3C

Expression

Insect

Mutation

CDC73(Y290A, Y293A)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1a-PAF1C(CTR9/CDC73/PAF1/LEO1/WDR61)

Plasmid

#231733

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Inserts

CTR9 (CTR9 Human)
CDC73 (CDC73 Human)
PAF1 (PAF1 Human)
LEO1 (LEO1 Human)
WDR61 (SKIC8 Human)

Use

Tags

3C-Strep(II) and Flag-3C

Expression

Insect

Mutation

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge