Addgene

We narrowed to 6,925 results for: Ski;

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Golgi-targeted optoPLD

Plasmid

#140058

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Use

Tags

CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPS gfp Blasti

Plasmid

#115804

Purpose

Express gfp driven by SFFV promoter

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gfp

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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FRB-myc

Plasmid

#20228

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FRB (MTOR Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T2098L point mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA3-AKAP1-mCherry-LOVPLD

Plasmid

#219558

Purpose

mitochondria-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

AKAP1-mCherry-LOVPLD

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-T2A-ACREB-WPRE

Plasmid

#40867

Depositor

Harold Gainer

Article

Lubelski et al Brain Res. 2012 Jan 6;1429:18-28. Epub 2

Insert

ACREB

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3-lyn10-mCherry- LOVPLD-P (LOVPLD)

Plasmid

#219555

Purpose

PM-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

lyn10-mCherry-LOVPLD

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-3a

Plasmid

#17994

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pCellFree_G01

Plasmid

#67135

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His

Expression

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pSETT4gap

Plasmid

#153413

Purpose

φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

Depositor

Joern Kalinowski

Article

Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

gapDH promoter from Eggerthella lenta

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)

Plasmid

#173456

Purpose

Contains mScarletH-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-H-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pscALPS_puro

Plasmid

#128504

Purpose

Encodes puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

Puromycin Resistance

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Cyclophilin A promoter

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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Golgi-targeted optodPLD

Plasmid

#140057

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Use

Tags

CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

Expression

Mammalian

Mutation

H170A (catalytic dead)

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-TREX1 g2 Cas9-T2A-mCherry

Plasmid

#164251

Purpose

Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgTREX1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pJEP230-Lenti-TRE3G-4xMyc-GluN2B(E1479Q)-WPRE-pA

Plasmid

#164795

Purpose

pLenti vector backbone designed to express 4XMyc Tagged-GluN2B(E1479Q) from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

4xMyc

Expression

Mutation

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL50005

Plasmid

#117536

Purpose

Level0 Golden Gate part: c-terminal tag, YFP

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

c-term tag YFP

Use

Synthetic Biology

Tags

Expression

Mutation

G65S, L68V, A72S, Y203T, L231H

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G04

Plasmid

#67138

Purpose

Gateway destination vector for cell-free expression with C-term eGFP 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

EGFP and HIS

Expression

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

Plasmid

#173453

Purpose

Contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Endosome-targeted optodPLD

Plasmid

#140055

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Use

Tags

2xFYVE, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pIRES_iSeroSnFR_cytosolic

Plasmid

#214132

Purpose

Fluorescent biosensor for intracellular serotonin (5-HT)

Depositor

Steffen Sinning

Article

Bukowski et al Research Square PREPRINT. 18 August 2023

Insert

iSeroSnFR_cytosolic

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-TREX1 g1 Cas9-T2A-mCherry

Plasmid

#164250

Purpose

Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgTREX1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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