We narrowed to 2,405 results for: dcas9

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pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

Plasmid

#84257

Purpose

Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation

Depositor

Stanley Qi

Publication

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9-ABI
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

Expression

Mammalian

Promoter

CAG and PGK

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCK389.AAV

Plasmid

#177327

Purpose

aTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expression

Depositor

James Carothers

Publication

Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

Inserts

SoxS
dCas9

Use

CRISPR

Tags

MCP (MS2 Coat Protein)

Mutation

R93A,S101A

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1

Plasmid

#121831

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

x-dCas9(3.7)/LSD1 (open)

Use

Gateway Cloning and Synthetic Biology

Tags

NLS

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS

Plasmid

#121828

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

x-dCas9(3.7) (open)

Use

Gateway Cloning and Synthetic Biology

Tags

NLS

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-DNMT1

Plasmid

#66818

Purpose

dCas9 fused to BFP and the human DNMT1 catalytic domain

Depositor

Yonglun Luo

Publication

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dCas9-BFP-DNMT1, catalytic domain (DNMT1 )

Expression

Mammalian

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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TDK promoter dCas9

Plasmid

#113657

Purpose

dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

Depositor

Jeffrey Barrick

Publication

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

TDK gene promoter and dCas9

Use

CRISPR

Expression

Bacterial

Promoter

TDK gene promoter

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pRN3P_EGFP_m6A_Tracer

Plasmid

#139403

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Publication

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

m6A-Tracer

Use

In vitro transcription

Tags

EGFP

Promoter

T3

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

Plasmid

#167898

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMRS-dCas9

Plasmid

#220193

Purpose

mini-Tn7 dCas9 expression vector

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

promoterless

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pRCA422 - pHR-UCOE-SFFV-VPH-dcas9-p2a-mCherry

Plasmid

#238165

Purpose

Express VPH-dCas9-P2A-mCherry in mammalian cells. Lentiviral

Depositor

Thomas Norman

Publication

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

VPH-dCas9-P2A-mCherry

Use

CRISPR and Lentiviral

Tags

mCherry

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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iF53 PB-Zim3-dCas9-mScarlet-Hygro

Plasmid

#204726

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

Plasmid

#106895

Purpose

Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation

Depositor

Stanley Qi

Publication

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9-ABI
GID1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

Expression

Mammalian

Promoter

CAG and PGK

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-KRAB-MeCP2-Puro

Plasmid

#242163

Purpose

Lentiviral expression of dCas9-KRAB-MeCP2 with puromycin reporter

Depositor

Amy Brock

Insert

dCas9-KRAB-MeCP2; T2A-puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pEf1a-superDn29-dCas9-P2A-GFP

Plasmid

#247156

Purpose

Mammalian expression of a human codon optimized engineered superDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

Depositor

Patrick Hsu

Publication

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

superDn29-dCas9-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

Promoter

Ef1a

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-HA-NLS-GFP4-VPR

Plasmid

#183924

Purpose

VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

Depositor

Karsten Rippe

Publication

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP4-VPR

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE3G-dCas9-VP64-MS2-VP64-ZsG

Plasmid

#192669

Purpose

Dox-inducible expression of dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

Depositor

Jeffrey Miner

Insert

dCas9-VP64, MS2-VP64, ZsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

TRE3G

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)

Plasmid

#160626

Purpose

TU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domains

Depositor

Diego Orzaez

Publication

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35s-dCas9:TV-Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64

Plasmid

#101110

Purpose

Encodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2

Depositor

Tudor Fulga

Publication

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(C)_DHFR-PCP-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJZC595

Plasmid

#62322

Purpose

Expresses MCP-VP64 and dCas9 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
dCas9

Tags

VP64

Expression

Yeast

Mutation

Has nuclease-inactivating mutations in the RuvC1 …

Promoter

Tdh3 and pAdh

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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TRE-dCas9(sa)-VP64-AAV

Plasmid

#213035

Purpose

dCas9-VP64 expressed under control of TRE

Depositor

Nadav Ahituv

Publication

Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

Insert

dCas9

Use

AAV and CRISPR

Tags

NLS and NLS-VP64

Promoter

TRE, miniCMV

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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