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pUC19-P70a-Top-T

Plasmid

#87312

Purpose

Expresses the "Top" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Top" transcript

Use

Synthetic Biology

Expression

Bacterial

Promoter

P70a

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B6-Leum

Plasmid

#44314

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B6-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B6-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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AEB-Theo27 pUC19

Plasmid

#71419

Purpose

This pUC19 vector contains a theophylline binding riboswitch, controlling the translation initiation rate of luciferase gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

Theo27 theophylline riboswitch

Use

Synthetic Biology

Promoter

ptac

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B12-Leum

Plasmid

#44320

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B12-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B12-Leum

Available Since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B5-Leum

Plasmid

#44313

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B5-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B5-Leum

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B10-Leum

Plasmid

#44318

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B10-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B10-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19-PolB-Arms1

Plasmid

#187197

Purpose

POLB gene homologous arm fragments to tag the POLB gene.

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2026 Apr 13;54(7):gka

Insert

Fragments of the PolB gene (POLB Human)

Use

Cloning vector

Promoter

LacZa

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pUC57_holD-GS12-holC

Plasmid

#254011

Purpose

Cloning vector containing synthetic ψ-GS12-χ fusion insert for subcloning and construct generation.

Depositor

Linda Bloom

Article

Padgett-Pagliai et al Nucleic Acids Res. 2026 May 20;54(10):gk

Insert

holD-GS12-holC

Expression

Bacterial

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD ΔS/GFP

Plasmid

#253144

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of spike, GFP is inserted at the locus of Spike

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD ΔS/GFP

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_ORF6-BirA

Plasmid

#253132

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), BirA fused to orf6, C-terminal

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD_ORF6-BirA (ORF6 SARS-CoV-2)

Tags

BirA

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_S Omicron BA.2

Plasmid

#253117

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), Wuhan backbone with Omicron BA.2 spike; chimeric

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

SARS-CoV-2 Omicron BA.2 spike

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_Δ3E_RABVG Δ67a

Plasmid

#253177

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; rabies glycoprotein replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with RABVG

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_S Omicron BA.1

Plasmid

#253116

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), Wuhan backbone with Omicron BA.1 spike; chimeric

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

SARS-CoV-2 Omicron BA.1 spike

Expression

Bacterial

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD1_XBB.1.5 S

Plasmid

#253119

Purpose

For recombinant virus rescue; encodes fragment D1 of SARS-CoV-2 (nt20950-25522), Omicron isolate XBB.1.5 Spike

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

SARS-CoV-2 Omicron XBB.1.5 spike

Expression

Bacterial

Mutation

S: F1089F

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-CC

Plasmid

#246943

Purpose

Plasmid strain containing pUC18T-mini-Tn7T-Gm-CC01 plasmid, which will insert a nonsense spacer version of the synthetic CRISPR-Cas system from P. aeruginosa PA14 into the genome at the att-Tn7 site.

Depositor

Tiffany Taylor

Article

Elliott et al Philos Trans R Soc Lond B Biol Sci. 2025

Insert

Type IF CRISPR-Cas system from Pseudomonas aeruginosa PA14

Use

Synthetic Biology

Expression

Bacterial

Promoter

Native promoters from PA14

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-Alpha 49

Plasmid

#244910

Purpose

Rep/Cap plasmid for producing variant AAV-hCA4-Alpha 49

Depositor

Viviana Gradinaru

Article

Lin et al Cell Rep. 2025 Oct 31;44(11):116419. doi

Insert

Synthetic construct producing AAV REP/CAP

Use

AAV

Expression

Mammalian

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pUC-GW-Kan-VPC

Plasmid

#244163

Purpose

Reporter for human p38alpha activation state by FRET

Depositor

Thomas Kuhlman

Insert

MAPK14/p38a (MAPK14 Human)

Use

Synthetic Biology

Tags

mCerulean3 and mVenus

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_21 tRNA genes

Plasmid

#242430

Purpose

In vitro transcription of 21 tRNAs after Nt.BspQI treatment. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene.

Depositor

Sebastian Maerkl

Article

Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

Insert

21 tRNA genes with a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene

Expression

Bacterial

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-AvrPWT4

Plasmid

#237516

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

AvrPwt4

Expression

Plant

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca_Reg2

Plasmid

#237513

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Reg2

Expression

Plant

Mutation

Removed binding region 2

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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