We narrowed to 838 results for: transfection

1. pcDNA3.1-GSDMD_Q29A

   Plasmid

   #224702

   Purpose

   Expression of mutated Gasdermin D_Q29A (GSDMD_Q29A) in transfected mammalian cell lines

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD_Q29A (GSDMD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q29A

   Promoter

   CMV

   Available Since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3.1-GSDMD_Q29A_H270A

   Plasmid

   #224703

   Purpose

   Expression of mutant Gasdermin D_Q29A_H270A (GSDMD_Q29A_H270A) in transfected mammalian cell lines

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD_Q29A_H270A (GSDMD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q29A and H270A

   Promoter

   CMV

   Available Since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pcDNA3.1-GSDMD_D275A

   Plasmid

   #224704

   Purpose

   Expression of mutated Gasdermin D_D275A (GSDMD_D275A) in transfected mammalian cell lines

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD_D275A (GSDMD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D275A

   Promoter

   CVM

   Available Since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pcDNA3.1-GSDMD_H270A_D275A

   Plasmid

   #224705

   Purpose

   Expression of mutated Gasdermin D_H270A_D275A (GSDMD_D275A) in transfected mammalian cell lines

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD_Q29A_H270A (GSDMD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H270A and D275A

   Promoter

   CMV

   Available Since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pHLsec hSOD1

   Plasmid

   #232481

   Purpose

   vector for transient transfection expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end

   Depositor

   Lukas Trantirek

   Article

   Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

   Insert

   Homo sapiens superoxide dismutase 1 (SOD1 Human)
   

   Tags

   DYKDDDDK (FLAG-tag)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+) EGFP-Rac1(G12C, K96H, P29S)

   Plasmid

   #224282

   Purpose

   Cell transfection and expression of Rac1 (G12C, K96H, P29S)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rac1 (RAC1 Human)
   

   Expression

   Mammalian

   Mutation

   G12C, K96H, P29S

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSmEn2ERT(#117)

   Plasmid

   #184079

   Purpose

   cyclofen-inducible chicken EN2 (wt) activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   6his-myc-chkEn2-ERT2
   

   Use

   Synthetic Biology

   Tags

   6his-Myc

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available Since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCSmEn2n4ERT(#234)

   Plasmid

   #184082

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   6his-myc-chkEn2(n4)-ERT2
   

   Use

   Synthetic Biology

   Tags

   6his-Myc

   Expression

   Mammalian

   Mutation

   chkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…

   Promoter

   sCMV+SP6

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCSmEn2SRERT(#231)

   Plasmid

   #184080

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   6his-myc-chkEn2(SR)-ERT2
   

   Use

   Synthetic Biology

   Tags

   6his-Myc

   Expression

   Mammalian

   Mutation

   chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …

   Promoter

   sCMV+SP6

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCSmEn25EERT(#232)

    Plasmid

    #184081

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    6his-myc-chkEn2(5E)-ERT2
    

    Use

    Synthetic Biology

    Tags

    6his-Myc

    Expression

    Mammalian

    Mutation

    chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

    Promoter

    sCMV+SP6

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+) K-Ras(G12C)

    Plasmid

    #224284

    Purpose

    Cell transfection and expression of K-Ras (G12C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    K-Ras (KRAS Human)
    

    Expression

    Mammalian

    Mutation

    G12C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5 CMV 3xFG eIF4G1

    Plasmid

    #158775

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EF1a_Puro_Telo_v2

    Plasmid

    #195139

    Purpose

    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Telomere (RTEL1 Human)
    

    Expression

    Mammalian

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSCVpuro-Mito-Pericam

    Plasmid

    #87381

    Purpose

    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.

    Depositor

    Björn Stork

    Article

    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

    Insert

    Mito-Pericam [ratiometric]
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    Retroviral 5' LTR promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+) K-Ras(WT)

    Plasmid

    #224283

    Purpose

    Cell transfection and expression of K-Ras

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    K-Ras (KRAS Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5 CMV 3xFG eIF4G1 +MIC

    Plasmid

    #158776

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (with microexon)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CMV-SNAP-hCB2_IRES_EGFP

    Plasmid

    #223506

    Purpose

    Expression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

    Depositor

    James Frank

    Article

    Kosar et al J Am Chem Soc. 2023 Jul 19;145(28):15094

    Insert

    SNAP-tag-CNR2 and EGFP (CNR2 Human, Synthetic)
    

    Tags

    SNAP-tag

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EF1a_Puro_Telo_v1

    Plasmid

    #195138

    Purpose

    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Telomere (RTEL1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5 frt/to N-BioTAP-C-BRD4-NUT

    Plasmid

    #171630

    Purpose

    Expresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.

    Depositor

    Chris French, Christopher A French

    Article

    Alekseyenko et al Proc Natl Acad Sci U S A. 2017 May 23;11

    Insert

    BRD4-NUT (BRD4, NUTM1 Human)
    

    Tags

    BioTAP

    Expression

    Mammalian

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5_miniTurbo-C12orf49

    Plasmid

    #155111

    Purpose

    transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    C12orf49 (SPRING1 Human)
    

    Tags

    miniTurbo

    Expression

    Mammalian

    Promoter

    CMV/TO inducible promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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