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pMS178

Plasmid

#215678

Purpose

Empty repair plasmid for ChrII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + loxP + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Promoter

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pMS114

Plasmid

#215677

Purpose

Empty repair plasmid for ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + loxN + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Promoter

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-Gal4-vp16

Plasmid

#171075

Purpose

Expresses Gal4-vp16 fusion protein in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4 DBD (NEWENTRY Budding Yeast)

Use

Tags

His and VP16 AD (413-490)

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCas9-EcRT-Z3-Hyg

Plasmid

#232095

Purpose

Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

Insert

Z3 promoter and Z3EV transcription factor

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Z3

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMS59

Plasmid

#215680

Purpose

Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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PP1 7-300

Plasmid

#26566

Depositor

Wolfgang Peti

Article

Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

Insert

PP1a (PPP1CA Human)

Use

Tags

His6, TEV cleavage site, and Thio-6

Expression

Bacterial

Mutation

Contains only aa 7-330

Promoter

Available since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only
pYPQAT57

Plasmid

#223429

Purpose

T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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LT2_12_GlyRNA

Plasmid

#177710

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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LT2_7_OxPro

Plasmid

#177709

Purpose

Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-TET3G-T2A-Puro

Plasmid

#158067

Purpose

Encodes the Tet3G transactivator and puromycin resistance.

Depositor

Adolfo Rivero-Muller

Article

Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

Insert

pCAG-TET3G-T2A-Puro

Use

AAV

Tags

puromycin reistance gene

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-3HA-kanMX6

Plasmid

#39295

Depositor

John Pringle, Jurg Bahler

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

3HA, A. gossypii translation elongation factor 1a…

Expression

Mutation

Promoter

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pBJ4

Plasmid

#167133

Purpose

A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Kanamycin promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pVDM1001

Plasmid

#134660

Purpose

CRISPR delivery and repair template delivery vector

Depositor

Willem van Schaik

Article

de Maat et al FEMS Microbiol Lett. 2019 Nov 1;366(22).

Insert

CRISPR guide RNA array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGE013

Plasmid

#108681

Purpose

All-in-one genome editing vector in M. polymorpha (hygromycin resistant)

Depositor

Shigeo Sugano, Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pYC1640

Plasmid

#158719

Purpose

CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

Sth1 sgRNA scaffold

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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JD631

Plasmid

#160399

Purpose

Binary vector pGWB14 including Vitis GRF4-GIF1 under 35S promoter/ Plant transformation

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

35S::vitis GRF4-GIF1 chimera

Use

Binary vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGH153_MS2-AIDΔ-Hygro

Plasmid

#85416

Purpose

lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AIDΔ and Hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-GST-kanMX6

Plasmid

#39293

Depositor

John Pringle, Jurg Bahler

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

Promoter

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pET28b-His tagged nuclear Pif1

Plasmid

#65047

Purpose

expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

Depositor

Jean-Baptiste Boulé, Virgina Zakian

Article

Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

Insert

Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)

Use

Tags

histag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PB-TO-hNGN2-BSD-mApple

Plasmid

#182308

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Inserts

hNGN2 (NEUROG2 Human)
bsd-T2A-mycNLS-mApple

Use

Tags

T2A-mycNLS-mApple

Expression

Mammalian

Mutation

Promoter

EF1a and TRE3G

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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