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pCMV-HA-mIFITM3-K24A

Plasmid

#58400

Purpose

Expresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysine 24 to Alanine

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pChlr-1SQ

Plasmid

#52139

Purpose

Encodes module 1 with amino acids 12S-16Q for recognition of nt 8A

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 25-60 (PUM1 Human)

Expression

Bacterial

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pChlr-2NQ

Plasmid

#52145

Purpose

Encodes module 2 with amino acids 12N-16Q for recognition of nt 7U

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 61-96 (PUM1 Human)

Expression

Bacterial

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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SL539

Plasmid

#49948

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

RHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)

Use

TALEN

Promoter

EF1a

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
pFA3

Plasmid

#46790

Purpose

To express ATPase inactive Fun30 in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Tags

V5

Expression

Yeast

Mutation

ATP-binding site mutated replaced lysin 603 by ar…

Promoter

PGAL1

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1 hSlp4-a V21A

Plasmid

#40064

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Tags

GST

Expression

Bacterial

Mutation

Valine 21 to Alanine

Promoter

Tac

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pCaSpeR-4 porcupine (genomic DNA)

Plasmid

#37376

Depositor

Norbert Perrimon

Article

Kadowaki et al Genes Dev. 1996 Dec 15;10(24):3116-28.

Insert

porcupine (por Fly)

Expression

Insect

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pEMS1097

Plasmid

#29028

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple201 (SLC6A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1408

Plasmid

#29205

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple201 (SLC6A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1673

Plasmid

#29290

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple201 (SLC6A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3 Flag Bmf (S74D)

Plasmid

#24259

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (S87D) (BMF Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 74 to Aspartic Acid

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL (S44A)

Plasmid

#24234

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44A) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed Serine 44 to Alanine

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
XE8 Xwnt-8 myc

Plasmid

#16868

Depositor

Randall Moon

Article

Christian et al Genes Dev. 1993 Jan. 7(1):13-28.

Insert

Xwnt-8 myc (wnt8a.L Frog)

Use

Xenopus expression

Tags

myc

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
NHR1+2-Flag pBacPAK8-3

Plasmid

#17324

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-MBP-ULK1-290-835-mcherry

Plasmid

#249733

Purpose

Expressing human ULK1-290-835 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

290-835

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pB-EFS-FastFUCCI-IRES-3xnls-mTagBFP2 (JDW 1515)

Plasmid

#242600

Purpose

A PiggyBac expression vector containing the core promoter from human EF1a driving FastFUCCI to label cell cycle state followed by an IRES-3xnls-mTagBFP2.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

human EF1a

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-ActinVhh-sfGFP-P2A-HA-tdiRFP-caax (JDW 1532)

Plasmid

#242606

Purpose

A CAGGS driven expression vector containing an actin nanobody fused to sfGFP to label actin followed by a HA tagged tdiRFP fused to a caax tag to label the cell membrane.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin Vhh-sfGFP-P2A-HA-tdiRFP-caax

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410)

Plasmid

#229807

Purpose

A CAGGS driven tet-on transactivator with an nls-EGFP reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

rtTA-IRES-nls-EGFP

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-MCS-c-Fos-b-globin (JDW 1237)

Plasmid

#229829

Purpose

A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

c-fos minimal promoter and b-globin intron

Use

Gateway Cloning

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pPICZ A-Strep-Opa1 (SB251)

Plasmid

#227600

Purpose

Inducible expression of Strep-tagged Opa1 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Tags

Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-960 only

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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