We narrowed to 10,959 results for: T7
-
Plasmid#183387PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertredesigned Caspase-4 (AA94-377) (CASP4 Human)
TagsHis-TEVExpressionBacterialMutationANR261ENP, D315H, M318K, T352KPromoterT7Available SinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET3a-FruK
Plasmid#186256PurposeOverexpression of FruK for purificationDepositorAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Kindlin
Plasmid#186136PurposeFor expression of recombinant Snap-labeled kindlin in bacteriaDepositorAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S7-S83C/Δ157-178
Plasmid#128592PurposeExpresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coliDepositorInsertS7 E.coli ribosomal protein (rpsG E.coli)
ExpressionBacterialMutationS83C, Δ157-178PromoterT7Available SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHBT-Mb(GDx-Clo_L10)
Plasmid#183406PurposeExpresses Monobody GDx-Clo_L10 in E. coliDepositorInsertHis tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)
TagsHis tag-Avi tag-TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K569A,K571A)-StrepII
Plasmid#174426PurposeExpresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteriaDepositorInsertDrp1
Tags6xHis(TEV) and StrepIIExpressionBacterialMutationK569A, K571APromoterT7Available SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term MBP SMT3 NikJC199U
Plasmid#174363PurposeExpression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
Tags8xHis, MBP, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sc NybbR eRF3∆165N (H348E)
Plasmid#175420PurposeEncodes His-MBP-TEV-ybbR-H348E eRF3∆165NDepositorInserteRF3 (SUP35 Budding Yeast)
TagsHis-MBP-TEV and ybbRExpressionBacterialMutation∆165N, H348EPromoterT7Available SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis
Plasmid#175788PurposePlasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184QPromoterT7Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K557A,K560A)-StrepII
Plasmid#174425PurposeExpresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteriaDepositorInsertDrp1
Tags6xHis(TEV) and StrepIIExpressionBacterialMutationK557A, K560APromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj12a
Plasmid#173141PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj12a (kcnj12a Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj16
Plasmid#173146PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj16a (kcnj16 Zebrafish)
UsePcr cloning vectorMutationStop codon removedPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-11a HIV-1 P6
Plasmid#154201PurposeBacterial expression of Gag P6 from HIV-1 HXB2 strainDepositorInsertGag P6 protein from HIV-1 clone HXB2
TagsGB1-6xHis TagExpressionBacterialPromoterT7Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ash1
Plasmid#169711PurposeBacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)DepositorInsertAsh1 (ASH1 Budding Yeast)
Tags6xHisExpressionBacterialMutationTruncation including encoding amino acids 420-500PromoterT7Available SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-N-wtBlc
Plasmid#168258PurposeN-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip proteinDepositorInsertN-fragment of wtBlc + Leucine Zipper
TagsHis-tagExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rDAD2 cPGFP(N180)
Plasmid#166001PurposeExpression of coding sequence in Escherichia coliDepositorInsertrDAD2 cPGFP(N180)
ExpressionBacterialMutationLSSmOrange- V49A (please see depositor comments)PromoterT7 promoterAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
OfCasp3b
Plasmid#166054Purposea caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6DepositorInsertOrbicella faveolata caspase-3b
TagsHis tagExpressionBacterialPromoterT7 lac promoterAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PaCasp3
Plasmid#166052Purposea caspase of Porites astreoides, which has higher sequence similarity with human caspase-3 but function similar to caspase-6DepositorInsertPorites astreiodes caspase-3
TagsHis tagExpressionBacterialPromoterT7 lac promoterAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc393
Plasmid#161953PurposeDemonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmidDepositorInsertpSB4A3-T-PT7-B30-mScarlet-I-3T
ExpressionBacterialPromoterPT7Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR
Plasmid#157672PurposeExpression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.DepositorInsertCohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
TagsddFLN4, ELP, Histag and ybbR tagExpressionBacterialPromoterT7Available SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only