We narrowed to 14,036 results for: TIM

1. pCA528 IST1 L328D, L355A (1-366)

   Plasmid

   #193040

   Purpose

   Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   L328D, L355A

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG CAPN7 V18D-OSF

   Plasmid

   #193042

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   V18D

   Promoter

   Chicken Beta-actin

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG CAPN7 L61D-OSF

   Plasmid

   #193043

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   L61D

   Promoter

   Chicken Beta-actin

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG CAPN7 F98D-OSF

   Plasmid

   #193044

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   F98D

   Promoter

   Chicken Beta-actin

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-CAPN7-V18D-mCherry

   Plasmid

   #193046

   Purpose

   Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with V18D (IST1-binding mutation) with C-terminal mCherry tag.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   V18D, siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Tight TRE

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLVX-CAPN7-C290S-mCherry

   Plasmid

   #193047

   Purpose

   Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C290S, siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Tight TRE

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCA528 CAPN7 V18D,F98D (1-165)

   Plasmid

   #193034

   Purpose

   Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   V18D, F98D, Residues 1-165

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PD2529 human beta1 ecto

   Plasmid

   #221398

   Purpose

   Mammalian expression of human integrin beta1 ectodomain

   Depositor

   Timothy Springer

   Article

   Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

   Insert

   integrin beta1 ectodomain (ITGB1 Human)
   

   Tags

   AVI tag, HRV3C cleavage site, basic coil, HA tag,…

   Expression

   Mammalian

   Mutation

   codon optimized for human mature _1 residues Q1 t…

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR-CLIP-CD28(CD45TM)

   Plasmid

   #223629

   Purpose

   Lentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   CLIP

   Mutation

   CD28 transmembrane domain replaced by CD45 transm…

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR-Spy-CD28(CD45TM)

    Plasmid

    #223624

    Purpose

    Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    Spytag003

    Mutation

    CD28 transmembrane domain replaced by CD45 transm…

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-SNAP-CD86(F56A)

    Plasmid

    #223609

    Purpose

    Lentiviral expression of human CD86 (F56A) with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD86 (CD86 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Mutation

    F56A

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR-SNAP-CD80(Y65A)

    Plasmid

    #223608

    Purpose

    Lentiviral expression of human CD80 (Y65A) with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD80 (CD80 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Mutation

    Y65A

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR-CLIP-CD28(SGGG)

    Plasmid

    #223607

    Purpose

    Lentiviral expression of human CD28 (SGGG) with CLIP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    CLIP

    Mutation

    MYPPPY mutated to SGGG

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPPI4-Strep-SNAP-CTLA-4-GCN4-His6

    Plasmid

    #223582

    Purpose

    Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CTLA4 (CTLA4 Human)
    

    Tags

    GCN4, His6 and Twin-Strep, SNAP

    Expression

    Mammalian

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPPI4-Strep-SNAP-CD80 (I92R)-His10

    Plasmid

    #223580

    Purpose

    Protein purification of human CD80 (I92R) with Twin-Strep, SNAP and 10xHis tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD80 (CD80 Human)
    

    Tags

    His10 and Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    I92R

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPPI4-Strep-SNAP-CD80-His10

    Plasmid

    #223579

    Purpose

    Protein purification of human CD80 with Twin-Strep, SNAP and 10xHis tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD80 (CD80 Human)
    

    Tags

    His10 and Twin-Strep, SNAP

    Expression

    Mammalian

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX459v3-eSpCas9(1.1)

    Plasmid

    #178800

    Purpose

    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PX459v3-SpCas9-HF1

    Plasmid

    #178801

    Purpose

    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTFG001-ACE2(WT)-HA

    Plasmid

    #191569

    Purpose

    ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag.

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    Angiotensin Converting Enzyme II (ACE2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    CMV

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Beta1 Q280* K418* full-length

    Plasmid

    #221406

    Purpose

    Mammalian expression of human integrin beta1 Q280* K418* full-length

    Depositor

    Timothy Springer

    Article

    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

    Insert

    integrin beta1 Q280* K418* full-length (ITGB1 Human)
    

    Tags

    CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

    Expression

    Mammalian

    Mutation

    codon-optimized mature sequence

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge