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AB.pCCL.sin.cPPT.GFP.miR-602.sensor.PGK.dNGFR.WPRE

Plasmid

#85865

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-620 Sensor (MIR620 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-583.sensor.PGK.dNGFR.WPRE

Plasmid

#85856

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-583 Sensor (MIR583 Human)

Use

Lentiviral

Promoter

PKG

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-301a-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85849

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-301a-3p (MIR301A Human)

Use

Lentiviral

Tags

-

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-218-1-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85892

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-218-1-3p Sensor (MIR218-1 Human)

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-214-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85898

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-214-3p Sensor (MIR214 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIP-Laforin Myc 3CS (C250S,C266S,C278S)

Plasmid

#84661

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc 3CS_new (C266S, C278S, C329S)

Plasmid

#84662

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pMCh-1469

Plasmid

#82419

Purpose

Plasmid for expression of NHA-tagged Nematostella vectensis GW182 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvGW182

Tags

NHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pMCh-1547

Plasmid

#82434

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis AGO1 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvAGO1

Tags

HA

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pMCh-1548

Plasmid

#82435

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis AGO2 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvAGO2

Tags

HA

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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FH95pUp97aGW (C3)

Plasmid

#74019

Purpose

lentiviral expression of Psd95 shRNA and SAP97alpha-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pET24a_CDP-CHase

Plasmid

#73161

Purpose

Expresses in E. coli, Bacillus cereus NP_831800 CDP-CHase

Depositor

Maurice Bessman, Sandra Gabelli

Article

Duong-Ly et al J Bacteriol. 2011 Jul;193(13):3175-85. d

Insert

pET24a

Expression

Bacterial

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
pES004 (pTet-qacR 5)

Plasmid

#69033

Purpose

qacR 5 downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Tags

His6 tag

Expression

Bacterial

Mutation

W61Y, E90Q, F102S, M116Q, Y119L, E120Q

Promoter

pTet

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pHL671

Plasmid

#62825

Purpose

Lim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Insert

gfp

Use

Synthetic Biology

Tags

glgC leader

Expression

Bacterial

Promoter

pCon

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL1017

Plasmid

#62828

Purpose

Lim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Insert

gfp

Use

Synthetic Biology

Tags

m8::glgC leader

Expression

Bacterial

Promoter

pConNoHind

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pET15b+PCNA3 T180C

Plasmid

#66181

Purpose

Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Mutation

T180C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 R112C

Plasmid

#66180

Purpose

Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Mutation

R112C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 E105C

Plasmid

#66176

Purpose

Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Mutation

E105C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA5woH

Plasmid

#65019

Purpose

Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, D160N, D165A, K19…

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM1-C103A

Plasmid

#58419

Purpose

Expresses murine IFITM1-C103A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 103 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only