We narrowed to 13,292 results for: cDNA

1. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)

   Plasmid

   #204696

   Purpose

   This plasmid allows for inducible expression of BRD4-NUT(deltaMIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-NUT(deltaMIN) (BRD4, NUTM1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   deletion of the amino acids 355-505 of NUT

   Promoter

   CMV

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)

   Plasmid

   #204695

   Purpose

   This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-NUT(MIN) (BRD4, NUTM1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   deletion of majority of NUT, retaining only amino…

   Promoter

   CMV

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT/TO-3*Flag-APEX2 N-term FBL

   Plasmid

   #187577

   Purpose

   Express FLAG epitope and APEX2-tagged FBL fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   FBL (FBL Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT/TO-3*Flag-APEX2 N-term COILIN

   Plasmid

   #187581

   Purpose

   Express FLAG epitope and APEX2-tagged COILIN fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   COILIN (COIL Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. (112) pcDNA3.1-GFP-Flag-Nup62-EPEA

   Plasmid

   #168101

   Purpose

   GFP and Flag tagged Nup62 with EPEA nanobody tag

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   GFP-Flag-Nup62-EPEA
   

   Use

   Tags

   EPEA, Flag, and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT/TO-3*Flag-APEX2 C-term EWSR1

   Plasmid

   #187586

   Purpose

   Express FLAG epitope and APEX2-tagged EWSR1 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PLKO-mcherry-luc-puro-Renilla cDNA

   Plasmid

   #29783

   Depositor

   Carl Novina

   Article

   Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

   Insert

   Renilla
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53

   Plasmid

   #187578

   Purpose

   Express FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   WRAP53 (WRAP53 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

   Plasmid

   #44027

   Depositor

   Celeste Simon

   Article

   Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

   Insert

   mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P405A, P530V, N851A

   Promoter

   CMV

   Available Since

   March 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

    Plasmid

    #164131

    Purpose

    Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E

    Plasmid

    #164013

    Purpose

    Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R464E

    Promoter

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

    Plasmid

    #163991

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro

    Plasmid

    #60909

    Purpose

    A minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    K560-E236A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3- myc NES-mCE(K294A) NLS

    Plasmid

    #82467

    Purpose

    Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NLS deleted from mouse Capping Enzyme (Rngtt Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of KRKY from 573-657

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

    Plasmid

    #87803

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1014 pcDNA3 T7 Akt1 K179M T308A S473A

    Plasmid

    #9031

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 K179M T308A S473A (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K179M T308A S473A

    Promoter

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2

    Plasmid

    #205160

    Purpose

    express β-ACTIN-3′UTR-8xMS2

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    β-ACTIN-3′UTR-24xMS2
    

    Use

    Tags

    tagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

    Plasmid

    #74309

    Purpose

    Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Rat, Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA 3.1(-) mouse C/EBP beta (LIP)

    Plasmid

    #12561

    Depositor

    Peter Johnson

    Insert

    CCAAT/Enhancer-binding Protein beta LIP (Cebpb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)

    Plasmid

    #49060

    Purpose

    Expresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP (chloride-sensitive)

    Expression

    Mammalian

    Mutation

    Cl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…

    Promoter

    CMV

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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