We narrowed to 6,879 results for: crispr cas9 plasmids

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CMV-CjABE8e

Plasmid

#189929

Purpose

Plasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

CjABE8e

Expression

Mammalian

Mutation

CjCas9 D8A

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)

Plasmid

#208289

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA; mutations in iSpyMac

Promoter

CMV and T7

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-gRNA

Plasmid

#158973

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

gRNA3

Expression

Mammalian

Promoter

U6

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Biomolecular FeedBack (FB)

Plasmid

#109391

Purpose

This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pJ23100mut-dCas9-T- phtpG1-sgRNA

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pJEP301-pAAV-CMV-MCS3-pA

Plasmid

#113676

Purpose

CMV driven Multi Cloning Site-3

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Promoter

Cytomegalo Virus(CMV)

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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LLP789_αGCN4-DNMT3A-CO

Plasmid

#211783

Purpose

SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-DNMT3A

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP306-pAAV-EFS-MCS3-pA

Plasmid

#113682

Purpose

EFS driven Multi Cloning Site-3.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-NRCH-PE2max-BSD

Plasmid

#191103

Purpose

Lentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PEmax

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Changed R221K, N394K, H840A from SpCas9-NRCH

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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LES2A-CRE

Plasmid

#167933

Purpose

Lentiviral CRE expression tracked by mScarlet-I fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Insert

mScarlet-P2A-CRE

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-BsaI_pJMP2776

Plasmid

#208890

Purpose

Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLY108

Plasmid

#130938

Purpose

Engineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB1

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY103

Plasmid

#130937

Purpose

Engineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA1

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLY76

Plasmid

#130963

Purpose

Engineered sgRNA-LEA2 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA2

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLY74

Plasmid

#130945

Purpose

Engineered sgRNA-G6 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-G6

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLY111

Plasmid

#130942

Purpose

Engineered sgRNA-LEB4 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB4

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLY110

Plasmid

#130940

Purpose

Engineered sgRNA-LEB3 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB3

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLY77

Plasmid

#130964

Purpose

Engineered sgRNA-LEB2 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB2

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY124

Plasmid

#130946

Purpose

Engineered sgRNA-G6-2MS2 (2 MS2 aptamers) generator circuit including promoter Pbad

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-G6-2MS2

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pbad

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY112

Plasmid

#130944

Purpose

Engineered sgRNA-LEB5 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB5

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY107

Plasmid

#130943

Purpose

Engineered sgRNA-LEA5 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA5

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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