We narrowed to 7,549 results for: URE

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pTS2366-Tier1-PhCMV_F-type

Plasmid

#169567

Purpose

Tier-1 vector encoding PhCMV-driven F-type transactivator domain (PhCMV-F-type-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven F-type VP16

Expression

Mammalian

Promoter

PhCMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX14-Tier1-PhCMV-mTFP1

Plasmid

#169511

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein mTFP1 (PhCMV-mTFP1-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven monomeric cyan fluorescent protein mTFP1

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

Plasmid

#169596

Purpose

Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

dead S.pyogenes Cas9

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2385-Tier1-PhCMV-fastFT

Plasmid

#169518

Purpose

Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT (PhCMV-fastFT-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven fluorescent timer, fast maturation time

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2384-Tier1-PhCMV-medFT

Plasmid

#169517

Purpose

Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT (PhCMV-medFT-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven fluorescent timer, medium maturation time

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX15-Tier1-PhCMV-mTurquoise2

Plasmid

#169512

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein mTurquoise2 (PhCMV-mTurquoise2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven monomeric cyan fluorescent protein mTurquoise2

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2408-Tier1-PhCMV-TEVsite

Plasmid

#169503

Purpose

Tier-1 vector encoding PhCMV-controlled tabacco etch virus protease recognition site (PhCMV-TEVsite-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven tobacco etch virus protease cleavage site

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1023-Tier1-PhCMV-p2A-PuroR

Plasmid

#169497

Purpose

Tier-1 vector encoding PhCMV-driven p2A sequence fused to a marker for puromycine selection (PhCMV-p2A-PuroR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-PuroR

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2402-Tier1-PhCMV-BlastR

Plasmid

#169494

Purpose

Tier-1 vector encoding PhCMV-driven blasticidin resistance gene BlastR (PhCMV-BlastR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven blasticidin-S deaminase

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1021-Tier1-PhCMV-PuroR

Plasmid

#169492

Purpose

Tier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven puromycin N-acetyl-transferase

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2409-Tier1-PhCMV-FURINsite

Plasmid

#169488

Purpose

Tier-1 vector encoding PhCMV-controlled FURIN protease recognition site (PhCMV-FURINsite-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Furin cleavage site

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2380-Tier1-PhCMV-TM

Plasmid

#169485

Purpose

Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) (PhCMV-TM-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Junctophilin 2 transmembrane domain (JPH2 Human)

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2379-Tier1-PhCMV-PEST

Plasmid

#169484

Purpose

Tier-1 vector encoding PhCMV-driven PEST tag for destabilization of proteins (PhCMV-PEST-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven PEST degradation tag

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVH9-Tier1-PhCMV-3xNLS

Plasmid

#169478

Purpose

Tier-1 vector encoding PhCMV-driven triple nuclear localization signal expression (PhCMV-3xNLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nuclear localization signal

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVH8-Tier1-PhCMV-NLS

Plasmid

#169477

Purpose

Tier-1 vector encoding PhCMV-driven nuclear localization signal expression (PhCMV-NLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nuclear localization signal

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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1137B=Hsp70Bb-Cas9

Plasmid

#153284

Purpose

The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)

Use

CRISPR

Tags

eGFP

Expression

Insect

Promoter

Dmel Hsp70Bb

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-HseIF4E_AF

Plasmid

#148669

Purpose

Bacterial Expression of HseIF4E

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4E (EIF4E Human)

Expression

Bacterial

Mutation

one silent mutation compared to the sequence of i…

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-BTLA (PD-1 ITIM)-mGFP

Plasmid

#180818

Purpose

Expression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

BTLA (PD-1 ITIM) (BTLA Human)

Use

Lentiviral

Tags

mEGFP

Mutation

replaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…

Promoter

SFFV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD-6R+6K

Plasmid

#178670

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-6R+6K (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

six Arg residues replaced with Lys

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD-3R+3K

Plasmid

#178669

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with three Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-3R+3K (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

three Arg residues replaced with Lys

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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