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pRSII305

Plasmid

#35439

Depositor

Steven Haase

Publication

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast integrating vector

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pFH2.1_mCherry

Plasmid

#205520

Purpose

Express mCherry (without export tag)

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-5xGCN4_v4-kif18b-24xPP7

Plasmid

#74927

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Publication

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

5xGCN4_v4-kif18b-24xPP7

Expression

Mammalian

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSII404

Plasmid

#35438

Depositor

Steven Haase

Publication

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast integrating vector

Promoter

None

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFCK(0.4)GW

Plasmid

#27229

Depositor

Pavel Osten

Publication

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Available since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe4_GCaMP6f

Plasmid

#213939

Purpose

AAV construct expressing GCaMP6f driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_GCaMP6f

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1 and AAV PHP.eB

Insert

GCaMP6f

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMaCTag-13

Plasmid

#119992

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry

Use

Pcr template

Tags

mCherry

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCM5.33

Plasmid

#19396

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

fbf-1 3'utr in pDONRP2RP3

Expression

Worm

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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Sp_PE

Plasmid

#212038

Purpose

Bacterial expression of the PE2 proteins for genome editing in E. coli

Depositor

Quanjiang Ji

Publication

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

PE2

Use

CRISPR

Promoter

L-arabinose-inducible promoter PBAD

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9

Plasmid

#128160

Purpose

Ptet-controlled expression of S. pyogenes Cas9

Depositor

Calvin Henard

Publication

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Promoter

Ptet

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Beclin-HA S-D

Plasmid

#46995

Purpose

Expresses Beclin1 in mammalian cells

Depositor

Kunliang Guan

Publication

Russell et al Nat Cell Biol. 2013 Jul;15(7):741-50. do

Insert

becn1

Tags

HA

Expression

Mammalian

Mutation

S14D

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEMS1277

Plasmid

#29140

Purpose

Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

CAG promoter

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,Δ42a] mut

Plasmid

#26577

Depositor

Diane Lipscombe

Publication

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR

Plasmid

#24549

Depositor

Thomas Wandless

Publication

Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

Insert

pADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)

Expression

Yeast

Mutation

1. Truncated version of ADH700 promoter (SbfI/Spe…

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only

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RBD#3L

Plasmid

#193020

Purpose

hnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

Depositor

Xue Gao

Publication

Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

Insert

Cas13a

Tags

hnRNP A1 RRM2

Expression

Bacterial

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3

Plasmid

#155095

Purpose

Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression

Depositor

Jason Moffat

Publication

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry

Plasmid

#155096

Purpose

Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mCherry expression

Depositor

Jason Moffat

Publication

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJFRC-nSyb-IVS-PhiC31

Plasmid

#133868

Purpose

Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.

Depositor

Tom Clandinin

Publication

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

PhiC31 Recombinase (int )

Expression

Insect

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SiC-V2-Cas9G7

Plasmid

#133043

Purpose

Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

Depositor

Sriram Neelamegham

Publication

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

Tet repressor

Use

Lentiviral

Tags

Cerulean

Available since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre

Plasmid

#125574

Purpose

Expresses Cre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Publication

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Cre

Expression

Mammalian

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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