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pAc5.1B-DmAdh-PTC64-V5His6_E

Plasmid

#146183

Purpose

Insect Expression of DmAdh-PTC64

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

DmAdh-PTC64 (Adh Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmAdh-V5His6_E

Plasmid

#146184

Purpose

Insect Expression of DmAdh

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

DmAdh (Adh Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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p32m3C Trx-CN

Plasmid

#196474

Purpose

Protein expression of Thiolredoxin-3C cleavage site-CarHCN

Depositor

Fei Sun

Article

Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

Insert

Trx-CN

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA-Target1 (MpRTN1)

Plasmid

#186291

Purpose

Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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MiniSOG-SpAGS-GFP

Plasmid

#185581

Purpose

for CALI

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

miniSOG-SpAGS

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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p09008_AF1-4+GFP5-7

Plasmid

#173715

Purpose

Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

Depositor

Johannes Kabisch

Article

Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

Inserts

hybrid GFP
mKate2

Use

E. coli cell free protein synthesis extracts

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-sgRNA-Target1 (MpRTN1)

Plasmid

#186295

Purpose

Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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KBB382

Plasmid

#185085

Purpose

HA-tagged POM152 with glycosylation sites mutated

Depositor

Ken Belanger

Article

Belanger et al Genetics. 2005 Nov;171(3):935-47. doi: 1

Insert

POM152

Tags

HA

Expression

Yeast

Mutation

Pom152 N280A, N398A, N???A, N???A = Pom152Dglyc

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only
pCAG-mTurbo-mito

Plasmid

#187177

Purpose

Express mitochondira-localized miniTurbo under the control of CAG promoter

Depositor

Isei Tanida

Article

Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

Insert

mtActA fused with miniTurbo (BirA mutant)

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-H2B-mTurbo

Plasmid

#187178

Purpose

Express histone H2B tagged with miniTurbo under the control of CAG promoter

Depositor

Isei Tanida

Article

Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

Insert

histone H2B fused with miniTurbo (BirA mutant)

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-ECL-3TM-hcvs-KKMP

Plasmid

#179641

Purpose

HCVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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IBAR-Cry2PHR

Plasmid

#176604

Purpose

Cry2PHR with N-terminal IBAR domain

Depositor

Robert Hughes

Article

Wurz et al J Biol Chem. 2022 Aug 18;298(10):102388.

Insert

IBAR-Cry2PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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C232-E08: attB2r-mTFP1-attB3

Plasmid

#162904

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIC3

Plasmid

#174036

Purpose

In vitro transcription of anti-CRISPR AcrIIC3 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC3

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIC1

Plasmid

#174035

Purpose

In vitro transcription of anti-CRISPR AcrIIC1 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC1

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBN079

Plasmid

#167960

Purpose

express E. coli E69 (O9a:K30) Wzc with Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN072

Plasmid

#167953

Purpose

express E. coli E69 (O9a:K30) Wzc with Y705E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y705E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y705E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN073

Plasmid

#167954

Purpose

express E. coli E69 (O9a:K30) Wzc with Y706E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y706E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y706E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN075

Plasmid

#167956

Purpose

express E. coli E69 (O9a:K30) Wzc with Y708E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y708E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y708E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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