We narrowed to 11,846 results for: NSI

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pUC19 KGA HA5'-mKO2-P2A-Zeo-3'

Plasmid

#110408

Purpose

Homology donor for glutaminase KGA isoform knock-in, mKO2-P2A-Zeo

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

CRISPR

Promoter

none

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc [ChromeT-GFP]

Plasmid

#153539

Purpose

AAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfKitlga

Plasmid

#140292

Purpose

Express soluble His-tagged zebrafish Kitlga in insect cells

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlga (kitlga Zebrafish)

Tags

6x His

Expression

Insect

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-MNX1wt-IDR

Plasmid

#145248

Purpose

Expresses fusion of GAL4 DNA-binding domain and MNX1wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MNX1wt-IDR (MNX1 Human)

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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RA35

Plasmid

#141126

Purpose

Expresses dimeric LacI repressor (LacI-11) under Rhl promoter

Depositor

Matthew Bennett

Article

Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

Inserts

dimeric LacI (LacI-11) (lacI Synthetic)
AiiA (AQ980_RS12585 )

Tags

ssrA degradation tag

Expression

Bacterial

Mutation

truncated/dimeric version of LacI (missing last 1…

Promoter

engineered rhl promoter (responds to C4-HSL) and …

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7RE Y165A

Plasmid

#139428

Purpose

GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

Changed tyrosine 165 to an alanine

Promoter

T7 promoter-1

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7RE P199A

Plasmid

#139427

Purpose

GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

Changed proline 199 to an alanine

Promoter

T7 promoter-1

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7RE I137A

Plasmid

#139319

Purpose

GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

Changed isoleucine 137 to an alanine

Promoter

T7 promoter-1

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMJA105

Plasmid

#128521

Purpose

Expresses amyloid human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme T70N (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-Ib

Plasmid

#121896

Purpose

Plasmid for making zebrafish apoA-Ib in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoA1b (apoa1b Zebrafish)

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoBb.2

Plasmid

#121899

Purpose

Plasmid for making zebrafish apoBb.2 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoBb.2 (apobb.2 Zebrafish)

Use

Unspecified

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-IVb.3

Plasmid

#121905

Purpose

Plasmid for making zebrafish apoA-IVb.3 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

apoa4b.3 (apoa4b.3 Zebrafish)

Use

Unspecified

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChromeT-GFP

Plasmid

#123316

Purpose

AAV-mediated expression of ChromeT-GFP under the Syn promoter.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

Promoter

Syn

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-IVa

Plasmid

#121902

Purpose

Plasmid for making zebrafish apoA-IVa in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoA4a (apoa4a Zebrafish)

Use

Unspecified

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-Ia

Plasmid

#121895

Purpose

Plasmid for making zebrafish apoA-Ia in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

apoA-Ia (apoa1a Zebrafish)

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(WT)

Plasmid

#112720

Purpose

Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(WT)

Tags

6His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P46A)

Plasmid

#112721

Purpose

This mutation (P46A) marks the first residue within the evolved region of U1A.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P46A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P46A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48T)

Plasmid

#112725

Purpose

This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48T)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48T

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 BICC FL

Plasmid

#112998

Purpose

For protein expression and purification of full-length Drosophila BicC

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length BicC (BicC Fly)

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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