We narrowed to 12,722 results for: CAR

1. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

   Plasmid

   #172316

   Purpose

   CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Fz4-GFP

   Plasmid

   #42197

   Depositor

   Jeremy Nathans, Robert Lefkowitz

   Article

   Chen et al Science. 2003 Sep 5;301(5638):1391-4.

   Insert

   Frizzled 4 (Fzd4 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPPEDs

   Plasmid

   #162469

   Purpose

   protoplast test vector of pPPED

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Inserts

   Cas9H840A
   attR gateway destination cassette
   

   Use

   Prime editing destination vector for transient ex…

   Tags

   MLV reverse transcriptase

   Expression

   Plant

   Mutation

   Change histidine 840 to Alanine

   Promoter

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgPBRM1-1- LentiCRISPRv2

   Plasmid

   #107403

   Purpose

   Lentiviral expression of Cas9 and gRNA targeting PBRM1

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   gRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgPBRM1-2- LentiCRISPRv2

   Plasmid

   #107404

   Purpose

   Lentiviral expression of Cas9 and gRNA targeting PBRM1

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   gRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJPT018-LAT_WT-HaloTag-3x FLAG

   Plasmid

   #202502

   Purpose

   Express WT-LAT

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

   Insert

   LAT (LAT Human)
   

   Use

   Tags

   HaloTag-3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLC242-GFP-metap2

   Plasmid

   #100518

   Purpose

   Expresses GFP tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   Metap2 (METAP2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T-1 3xFlag-STAT1

   Plasmid

   #82722

   Purpose

   Expresses human STAT1

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   STAT1 (STAT1 Human)
   

   Use

   Tags

   3xFLAG and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

9. RV-Cag-Dio-EYFP-P2A-Mafb

   Plasmid

   #98175

   Purpose

   Retroviral vector encoding Cre-dependent expression of EYFP-P2A-Mafb

   Depositor

   Oscar Marin

   Article

   Mi et al Science. 2018 Feb 22. pii: science.aar68

   Insert

   Mafb (Mafb Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV10.KH

    Plasmid

    #63208

    Purpose

    pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbE1k-3

    Plasmid

    #71722

    Purpose

    tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

    Depositor

    Taek Soon Lee

    Article

    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

    Inserts

    dihydropterine reductase (QDPR Human)
    pterin-4a-carbinolamine dehydratase (PCBD1 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTrc

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. MoFGFR1c-ires neo

    Plasmid

    #86490

    Purpose

    mamalian cell expression of FGFR1c

    Depositor

    David Ornitz

    Article

    Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

    Insert

    FgfrR1 IIIc 15D8 (Fgfr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Moloney murine leukemia virus LTR

    Available since

    Feb. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2-hDKK2-flag

    Plasmid

    #15495

    Depositor

    Sergei Sokol

    Article

    Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

    Insert

    DKK2 (DKK2 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

14. nLuc-ΔECD-PTHR1

    Plasmid

    #190011

    Purpose

    Expresses the Nanoluciferase fused to the human parathyroid hormone receptor type 1 that is missing the extracellular domain

    Depositor

    Sam Gellman

    Article

    Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

    Insert

    nLuc-ΔECD-PTHR1 (PTH1R Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCD564

    Plasmid

    #153026

    Purpose

    dxCas9(3.7), MCP-SoxS(R93A/S101A)

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dxCas9(3.7), MCP-SoxS(R93A/S101A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Available since

    Aug. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX302-MKP5-V5 puro

    Plasmid

    #87770

    Purpose

    Expresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-FLAG-Sestrin1 isoform1

    Plasmid

    #72594

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN1 (SESN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

18. FLAG-TKFC in pLJM60

    Plasmid

    #154206

    Purpose

    Lentiviral vector for stable expression of TKFC

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    TKFC (DAK Human)
    

    Use

    Lentiviral

    Tags

    N terminal FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBRPyCAG-hKlf2-DsRed-Ip

    Plasmid

    #26277

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Insert

    Klf2 (KLF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

    Plasmid

    #174892

    Purpose

    amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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