We narrowed to 14,251 results for: TIM

Showing: 6721 - 6740 of 14251 results

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pCMV5-MLLn-CAGEn-NES (Nrdj1)

Plasmid

#241412

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Figure 2 & Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

MLLn-CAGEn-NES (Nrdj1) (KMT2A Human, Synthetic)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-MLLn-CAGEn-NES (Npu)

Plasmid

#241411

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

MLLn-CAGEn-NES (Npu) (KMT2A Human, Synthetic)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-AMLn-CAGEn-NES (Nrdj1)

Plasmid

#241408

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product AML1-ETO, Figure 2 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

AMLn-CAGEn-NES (Nrdj1) (RUNX1 Human, Synthetic)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-NGN3-linker-3xFLAG-Dendra2-HA

Plasmid

#234887

Purpose

Expresses human NGN3 in mammalian cells directly fused to Dendra2 fluorescent protin plus FLAG and HA tags

Depositor

Nancy Papalopulu

Article

Miller et al Dev Cell. 2025 Sep 22;60(18):2518-2532.e

Insert

NGN3-3xFLAG-Dendra2-HA (NEUROG3 Human)

Tags

3xFLAG, Dendra2 and HA

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-AMLn-CAGEn-NES (Npu)

Plasmid

#241407

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product AML1-ETO, Extended Figure 3 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

AMLn-CAGEn-NES (Npu) (RUNX1 Human, Synthetic)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_G-PTEN

Plasmid

#227432

Purpose

Expresses the G-PTEN sensor under a CMV promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN (Pten Rat)

Tags

mEGFP and sREACh

Expression

Mammalian

Mutation

R14G

Promoter

CMV

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBC001 v3

Plasmid

#161711

Purpose

CMVp-EGFP-[barcode cloning site]-PGKp-Puro-WPRE

Depositor

Nozomu Yachie

Article

Nakayama et al Sci Immunol. 2024 May 24;9(95):eade3814.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

CMV Promoter

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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Donor-attp-u6-blank-sgrna-backbone

Plasmid

#234076

Purpose

Blank donor for Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

Depositor

Gary Hon

Article

Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

Insert

sgRNA-Blank

Use

Lentiviral

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_R-PTEN_4A

Plasmid

#227435

Purpose

Expression of the R-PTEN sensor with a 4A mutation

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

R-PTEN 4A (Pten Rat)

Tags

mCyRFP2, mMaroon

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CAG_G-PTEN

Plasmid

#227436

Purpose

Expresses the G-PTEN sensor under a CAG promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN sensor, mEGFP-PTEN-sREACh (Pten Rat)

Tags

mEGFP, and sREACh

Expression

Mammalian

Mutation

R14G

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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PX330-PTEN

Plasmid

#227440

Purpose

Cas9 expression and a gRNA targeting Exon 6 of mouse PTEN

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

spCas9 and gRNA for mouse PTEN (Pten )

Expression

Mammalian

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_G-PTEN_4A

Plasmid

#227434

Purpose

Expresses the G-PTEN sensor with a 4A mutation

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN 4A (Pten Rat)

Tags

mEGFP and sREACh

Expression

Mammalian

Mutation

4A mutation.

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCpep-E1GFP

Plasmid

#233884

Purpose

Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

Proinsulin-E1GFP (Ins2 Mouse)

Expression

Mammalian

Mutation

Inserted E1GFP into C-peptide of mouse Ins2 codin…

Promoter

CMVF

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1 L328D, L355A (1-366)

Plasmid

#193040

Purpose

Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

L328D, L355A

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG CAPN7 V18D-OSF

Plasmid

#193042

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Mutation

V18D

Promoter

Chicken Beta-actin

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG CAPN7 L61D-OSF

Plasmid

#193043

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Mutation

L61D

Promoter

Chicken Beta-actin

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG CAPN7 F98D-OSF

Plasmid

#193044

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Mutation

F98D

Promoter

Chicken Beta-actin

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CAPN7-V18D-mCherry

Plasmid

#193046

Purpose

Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with V18D (IST1-binding mutation) with C-terminal mCherry tag.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

V18D, siRNA resistant to: GCACCCAUACCUUUACAUU

Promoter

Tight TRE

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CAPN7-C290S-mCherry

Plasmid

#193047

Purpose

Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

C290S, siRNA resistant to: GCACCCAUACCUUUACAUU

Promoter

Tight TRE

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CAPN7 V18D,F98D (1-165)

Plasmid

#193034

Purpose

Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

V18D, F98D, Residues 1-165

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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