165,567 results

1. pEFBOS_CreIRESpuro

   Plasmid

   #183812

   Purpose

   Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

   Depositor

   Ed Stanley

   Article

   Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

   Insert

   Cre recombinase
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 - hSRSF6

   Plasmid

   #120419

   Purpose

   overexpression of human SRSF6

   Depositor

   Gillian Bates, Andreas Neueder

   Article

   Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

   Insert

   SRSF6 (SRSF6 Human)
   

   Expression

   Mammalian

   Promoter

   CMV immediate/early

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-4mtD3cpv

   Plasmid

   #36324

   Depositor

   Roger Tsien, Amy Palmer

   Article

   Palmer et al Chem Biol. 2006 May;13(5):521-30.

   Insert

   4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
   

   Tags

   First 29 aa of human COX subunit 8a

   Promoter

   CMV

   Available Since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHTNW

   Plasmid

   #136403

   Purpose

   Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

   Plasmid

   #217635

   Purpose

   AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

   Depositor

   Martin Kampmann

   Article

   Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

   Insert

   mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
   

   Use

   AAV

   Promoter

   EF1alpha and mU6

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-DAAO

   Plasmid

   #60344

   Purpose

   Yeast D-Amino acid oxidase from Rhodotorula gracilis, optimized for mammalian expression.

   Depositor

   Vsevolod Belousov

   Article

   Matlashov et al Antioxid Redox Signal. 2014 Mar 1;20(7):

   Insert

   DAAO
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-polyQ74-NLS

   Plasmid

   #231892

   Purpose

   Forms nuclear HTT partial exon 1 Q74-NLS aggregates

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q74-NLS (HTT Human)
   

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Mutation

   CAG repeat expansion

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-EGFP-C1-HsPatL1_I

   Plasmid

   #146547

   Purpose

   Mammalian Expression of HsPatL1

   Depositor

   Elisa Izaurralde

   Article

   Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

   Insert

   HsPatL1 (PATL1 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pM1s3AsG

   Plasmid

   #137921

   Purpose

   E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Insert

   Superfolder GFP
   

   Use

   Synthetic Biology; For use with e. coli nissle in…

   Expression

   Bacterial

   Promoter

   J23101

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19/PLIN2-GFP

    Plasmid

    #202194

    Purpose

    knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    Cloning vector

    Tags

    GFP knock-in

    Promoter

    lac

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Flpe

    Plasmid

    #13787

    Purpose

    Mammalian expression of enhanced form of Flp recombinase with C-terminal Myc tag

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Flpe
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-HA-mSTING

    Plasmid

    #214157

    Purpose

    Transient expression of STING

    Depositor

    Nan Yan

    Article

    Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

    Insert

    STING (Sting1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW57.1 N-term GFP tTA

    Plasmid

    #107551

    Purpose

    All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

    Depositor

    David Sabatini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP (optional)

    Expression

    Mammalian

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPW3758 : CMVd3-HsIRE1-HaloTag

    Plasmid

    #185680

    Purpose

    Low-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Human, Synthetic)
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJQ200SK

    Plasmid

    #78497

    Purpose

    Suicide vector for gene replacement

    Depositor

    Michael Hynes

    Article

    Quandt et al Gene. 1993 May 15;127(1):15-21.

    Type

    Empty backbone

    Use

    Suicide plasmid

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR-ABCB1_STOP

    Plasmid

    #221424

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

    Insert

    ABCB1 (ABCB1 Human)
    

    Expression

    Mammalian

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-UTX-F

    Plasmid

    #17438

    Purpose

    Expression of UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    UTX (KDM6A Human)
    

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF2547

    Plasmid

    #144039

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CEBPD (CEBPD Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV Myc T58A puro

    Plasmid

    #18773

    Depositor

    Scott Lowe

    Article

    Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

    Insert

    myc T58A (MYC Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCIneo RYR-1

    Plasmid

    #75113

    Purpose

    Clone encoding rabbit ryanodine receptor in mammalian expression vector

    Depositor

    Robert Dirksen, Paul Allen

    Article

    Nakai et al J Biol Chem. 1998 May 29;273(22):13403-6

    Insert

    Ryanodine receptor 1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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