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pPOTv7-blast-mScarlet

Plasmid

#179818

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-mScarletI

Plasmid

#179820

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-g418-mNG

Plasmid

#179821

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-g418-mScarletI

Plasmid

#179822

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-g418-mScarlet

Plasmid

#179823

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-blast-10Ty

Plasmid

#179807

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xTy

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-hygro-10Ty

Plasmid

#179808

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xTy

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-blast-SNAPtag

Plasmid

#179786

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

SNAP

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-blast-birA*

Plasmid

#179788

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

birA

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD540 Reporter Template v3

Plasmid

#138750

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation. BsaI sites produce non-palindromic sticky ends.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPSG830

Plasmid

#158601

Purpose

tNCS-ePTS1 (2micron)

Depositor

John Dueber

Article

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Insert

pTDH3-tNCS-ePTS1

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pVL23 - pVND7

Plasmid

#116019

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pVND7

Use

Unspecified

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSW618 - pAHP6

Plasmid

#115991

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pAHP6

Use

Unspecified

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pVL15 - tSCR

Plasmid

#116030

Purpose

entry vector for GreenGate cloning method, terminator

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

tSCR

Use

Unspecified

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAW203

Plasmid

#113243

Purpose

E. coli expression vector for IHFa E10A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa E10A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

E10A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Bxbi+PhiC31 GFP Bandpass BAC Reporter

Plasmid

#78218

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available Since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxbi+TP901 GFP Bandpass BAC Reporter

Plasmid

#78229

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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2xNLS-pMJ915v2-sfGFP

Plasmid

#88920

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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1xNLS-pMJ915v2-sfGFP

Plasmid

#88919

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pKF296

Plasmid

#74773

Purpose

template for C-terminal TEV-site + 2x V5-tag (short version), Blasticidin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

TEV- 2xV5 tag (short version)

Expression

Insect

Available Since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

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