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pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 luciferase

Plasmid

#26612

Depositor

Jie Chen

Article

Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

Insert

firefly luciferase

Expression

Mammalian

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-RIPK3

Plasmid

#78815

Purpose

To overexpress RIPK3 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Nst-CAG-DEST

Plasmid

#80489

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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single polypeptide FPX biosensor for calcium ion

Plasmid

#60887

Purpose

FPX biosensor

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

single polypeptide FPX biosensor for calcium ion

Tags

HindIII site

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2A

Plasmid

#24180

Purpose

Mammalian expression of human JMJD2A with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2A (KDM4A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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HA-Elongin B-pcDNA3.1(+)-Zeo

Plasmid

#20000

Depositor

William Kaelin

Article

Ohh et al Nat Cell Biol. 2000 Jul . 2(7):423-7.

Insert

transcription elongation factor B (ELOB Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wild type

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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hE-cadherin/Δβ-catenin-pcDNA3

Plasmid

#45772

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin/Δβ-catenin (CDH1 Human)

Expression

Mammalian

Mutation

Ends at amino acid 844. Last 35 amino acids dele…

Promoter

CMV

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only

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pGWB555

Plasmid

#74885

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1

Plasmid

#17791

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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NPY-mCherry

Plasmid

#67156

Purpose

Expresses NPY-mCherry

Depositor

Wolfhard Almers

Article

Barg et al Proc Natl Acad Sci U S A. 2010 Nov 30;10

Insert

NPYmCherry (NPY Human)

Tags

mCherry

Expression

Mammalian

Promoter

cmv

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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HA-EglN3-pcDNA3

Plasmid

#18960

Depositor

William Kaelin

Article

Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

Insert

Egg laying Nine - 3 (EGLN3 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pJMK074: CMV QuasAr2-TS-GCaMP6f

Plasmid

#72303

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Article

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAB1

Plasmid

#62547

Purpose

broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

Depositor

Michael Franklin

Article

Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

Insert

eGFP

Use

Broad host range plasmid

Expression

Bacterial

Promoter

pTrc

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp10-2xStrep-IRES-Puro

Plasmid

#141376

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp10 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 S

Plasmid

#149329

Purpose

Gateway-compatible Entry vector, with insert of the spike (S) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S (S SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHR SFFVp YFP-PIF-SOS2cat

Plasmid

#50851

Purpose

Optogenetic Ras activation system; cytoplasmic component

Depositor

Wendell Lim

Article

Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

Inserts

SOS2cat (SOS2 Human)
PIF6 1-100

Use

Lentiviral

Tags

YFP

Mutation

residues 566–1049

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Cdc42-wt

Plasmid

#12972

Depositor

Gary Bokoch

Insert

Cdc42 (CDC42 Human)

Tags

Myc

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pLuc-CDS

Plasmid

#42100

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Renilla luciferase

Expression

Mammalian

Promoter

SV40

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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mCD8-GFP in pUAST

Plasmid

#17746

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

mouse CD8 (Cd8a Mouse)

Tags

GFP

Expression

Insect

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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