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Showing: 6761 - 6780 of 19450 results

  4. pcDNA3-ΔN-RNMT

    Plasmid
    #112708
    Purpose
    Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.
    Depositor
    Daniel Schoenberg
    Publication
    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNMT (RNMT Human)
    Tags
    FLAG and HIV Rev nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    deleted amino acids 2-120, mutated active-site D2…
    Promoter
    CMV
    Available since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112708-plasmid-map-sequence-id-217040

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  5. B52 + CHEK2 sgSTOP

    Plasmid
    #100712
    Purpose
    B52 plasmid expressing CHEK2 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
    Depositor
    Alberto Ciccia
    Publication
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgSTOP targeting CHEK2 (cloned using BbsI) (CHEK2 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoters
    Available since
    Sept. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    100712-plasmid-map-sequence-id-193017

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  7. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid
    #159676
    Purpose
    Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.
    Depositor
    Omar Akbari
    Publication
    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1
    Insert
    pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    Expression
    Insect
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159676-plasmid-map-sequence-id-314890

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  8. AAV-KrasG12S/sgKras/Cre

    Plasmid
    #99853
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Publication
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99853-plasmid-map-sequence-id-193755

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  9. AAV-KrasG13D/sgKras/Cre

    Plasmid
    #99857
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Publication
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99857-plasmid-map-sequence-id-193798

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  12. pPL001

    Plasmid
    #184750
    Purpose
    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.
    Depositor
    Jef Boeke
    Publication
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pEF1a-eGFP-T2A-BSD-bGHpA
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    184750-plasmid-map-sequence-id-365374

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  13. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135014-plasmid-map-sequence-id-267599

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  16. pMVRha

    Plasmid
    #229553
    Purpose
    Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.
    Depositor
    Sandy Schmidt
    Publication
    Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    229553-plasmid-map-sequence-id-461178

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  17. pQE80l B-CN-B

    Plasmid
    #196477
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN
    Depositor
    Fei Sun
    Publication
    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1
    Insert
    B-CN-B
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196477-plasmid-map-sequence-id-387241

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Showing: 6761 - 6780 of 19450 results