We narrowed to 5,906 results for: pcDNA 3.1

1. pcDNA3.1-MS2-HA-HsRck_AC

   Plasmid

   #148417

   Purpose

   Mammalian Expression of HsRck

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsRck (DDX6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Gαq*-BERKY3

   Plasmid

   #158217

   Purpose

   BRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-3XHA-p53 Δ1-42

   Plasmid

   #196269

   Purpose

   Mammalian expression of 3xHA tagged p53 Δ1-42 (ΔTAD1)

   Depositor

   Laura Attardi

   Article

   Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

   Insert

   p53 Δ1-42 (Trp53 Mouse)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   deleted amino acids 1 to 42

   Promoter

   CMV

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-3XHA-Zmat3

   Plasmid

   #167343

   Purpose

   Expresses HA-tagged Zmat3

   Depositor

   Laura Attardi

   Article

   Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

   Insert

   Zinc finger matrin-type 3 (Zmat3 Mouse)
   

   Use

   Tags

   3HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-IDH2-R172K

   Plasmid

   #87927

   Purpose

   Expresses IDH2 (R172K mutant) in mammalian cells

   Depositor

   Reproducibility Project Cancer Biology

   Article

   Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

   Insert

   isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   R172K

   Promoter

   CMV

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-TYRO3-V5/HIS

   Plasmid

   #200118

   Purpose

   Expression of human TYRO3 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human TYRO3 receptor tyrosine kinase (TYRO3 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-MS2-HA-HshnRNPE1_Z

   Plasmid

   #148115

   Purpose

   Mammalian Expression of HshnRNPE1

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPE1 (PCBP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-3C-sGFP1-10_pcDNA3.1(-)

   Plasmid

   #182931

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ELP-TEMP/pcDNA3.1(-)

   Plasmid

   #178434

   Purpose

   Genetically encoded ratiometric fluorescent thermometer

   Depositor

   Takeharu Nagai

   Article

   Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

   Insert

   ELP-TEMP
   

   Use

   Tags

   No tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 3xFlag IFIT3

    Plasmid

    #53553

    Purpose

    mammalian expression of IFIT3

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT3 (IFIT3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1 Flag hHMGB1

    Plasmid

    #31609

    Depositor

    Yasuhiko Kawakami

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1+/-FLI1-C‐(K)‐DYK

    Plasmid

    #129497

    Purpose

    Mammalian expression of human FLI1

    Depositor

    Marjorie Brand

    Article

    Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1.Gαs-LgB113

    Plasmid

    #134362

    Purpose

    Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαs-LgB113 (GNAS Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Kv7.4 (1-695)/pcDNA3.1

    Plasmid

    #111453

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    8xHis and EGFP

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-695)

    Promoter

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-hRIPK1-Flag

    Plasmid

    #112487

    Purpose

    Mammalian expression of Flag-hRIPK1. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+) K-Ras(G12C)

    Plasmid

    #224284

    Purpose

    Cell transfection and expression of K-Ras (G12C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    K-Ras (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-CHRNA7-mCherry

    Plasmid

    #62632

    Purpose

    mammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    hAlpha7 (CHRNA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mCherry inserted into M3-M4 loop

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-MS2-HA-HsSmg5_T

    Plasmid

    #147552

    Purpose

    Mammalian Expression of HsSmg5

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg5 (SMG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-Cyto-CaNARi

    Plasmid

    #64849

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Use

    Tags

    Cerulean, Nuclear export signal (NES), and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-RET-V5/HIS

    Plasmid

    #205606

    Purpose

    Expression of human RET receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RET receptor tyrosine kinase (RET Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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