We narrowed to 156,007 results for: ins
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Plasmid#228418PurposeExpression of NIT-9 with moco resulfurase linkage region in N. crassa at the his-3 locusDepositorInsertNIT-9 (GPHN Human)
UseProtein expression in n. crassaMutationremoval of the linkage region and insertion of th…Available SinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIS-rPAM3
Plasmid#145803Purposeexpresses rat PAM3 in mammalian cellsDepositorInsertrat PAM3
ExpressionMammalianMutationSee depositor comments belowPromoterCMVAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 1-16 3x FLAG
Plasmid#111966PurposeHuman HEATs 1-16 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 1-16 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 1-16 insertedAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 1-12 3xFLAG
Plasmid#111965PurposeHuman HEATs 1-12 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 1-12 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 1-12 insertedAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 12-20 3xFLAG
Plasmid#111972PurposeHuman HEATs 12-20 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 12-20 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 12-20 insertedAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 5-12 3xFLAG
Plasmid#111968PurposeHuman HEATs 5-12 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 5-12 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 5-12 insertedAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 5-20 3xFLAG
Plasmid#111971PurposeHuman HEATs 5-20 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 5-20 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 5-20 insertedAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 1-5 3xFLAG
Plasmid#111963PurposeHuman HEATs 1-5 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 1-5 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 1-5 insertedAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 5-16 3xFLAG
Plasmid#111969PurposeHuman HEATs 5-16 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 5-16 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 5-16 insertedAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCY 3120-04
Plasmid#36238DepositorInsertTRP1
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yECitrine-13Myc-TRP1Available SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYMA3_SidK_GFP
Plasmid#184276PurposeLegionella pneumophila SidK (1-278) - GFPDepositorAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMSP1E1D1
Plasmid#161605PurposeBacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formationDepositorInsertMSP1E1D1
Tags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeat of h…PromoterT7Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pDonor14-N7HELIX-KanR
Plasmid#190663PurposeKanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequenceDepositorInsertKanR, R6K origin of replication
ExpressionBacterialPromoterNAAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShHELIX-KanR (CJT70)
Plasmid#181793PurposepDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
ExpressionBacterialAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAraH6HATT
Plasmid#63901PurposeFor expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.DepositorInsertTT Fab
Tags6xHis and HA tagExpressionBacterialPromoteraraBADAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYMA1_SidK_mCH
Plasmid#184906PurposeLegionella pneumophila SidK (1-278) - mCherryDepositorAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E2D1
Plasmid#161606PurposeBacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formationDepositorInsertMSP1E2D1
Tags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeat of h…PromoterT7Available SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD284
Plasmid#66825PurposeTagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized TagRFP-TExpressionWormAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 integrase F185K/C280S in pET-15b
Plasmid#61669PurposeHIV-1 integrase F185K/C280S in pET-15bDepositorInsertHIV-1 integrase F185K/C280S
UseSynthetic BiologyTagsHisPromoterT7 promotorAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDD285
Plasmid#66826PurposemKate2^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmKate2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized mKate2ExpressionWormAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVTU_260_BirA
Plasmid#63682PurposeEncodes E. coli biotin ligase gene (BirA) for expression in yeast cellsDepositorInsertBirA
Tags6X Histidine and TEV cleavage siteExpressionYeastPromoterADH1/yeastAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TAA
Plasmid#208187Purposenonsense mistranslation, TAA stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterExpressionBacterialMutationInsertion 6TAA*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cmv d0 dPspCas13b-GGS-NYTHDF1
Plasmid#119855PurposeCmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)DepositorInsertdCas13b-GGS-NYTHDF1
UseCRISPRExpressionMammalianAvailable SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSP2
Plasmid#26282DepositorInsertMSP2
Tags6-HisExpressionBacterialMutationsynthetic gene of dimer of deletion mutant (1-43)…Available SinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rabx6 WT
Plasmid#53479DepositorAvailable SinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TAG
Plasmid#208188Purposenonsense mistranslation, TAG stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterExpressionBacterialMutationInsertion 6TAG*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TGA
Plasmid#208189Purposenonsense mistranslation, TGA stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterExpressionBacterialMutationInsertion 6TGA*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TGATC
Plasmid#208190Purposenonsense mistranslation, TGA stop variant 2DepositorInsertsGFPmut2
mScarlet-I
UseReporterExpressionBacterialMutationInsertion 6TGA*(stop) 7TCT(Ser)PromoterPtet+dnaK P1Available SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only