We narrowed to 2,263 results for: EXO

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pET32a-HD39Q

Plasmid

#11514

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 39 glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 39 glutamines, vector m…

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pCA-G-intron-G

Plasmid

#40027

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP exon 1
GFP exon 2
beta-globin intron

Expression

Mammalian

Mutation

insertion of a loxP site, nucleotides 1-274, and …

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pX330 _CRISPIE donor B8

Plasmid

#172849

Purpose

DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B8 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-2 sgRNA and CRISPIE designer exon (phase 1-1) encoding mEGFP

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-EML4-ALK variant 3a G1202R

Plasmid

#183830

Purpose

Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cells

Depositor

Aaron Hata

Article

Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

Inserts

ALK Receptor Tyrosine Kinase (ALK Human)
EMAP Like 4 (EML4 Human)

Use

Lentiviral

Tags

The fusion protein of EML4 exon 1-6a and ALK exon…

Expression

Mammalian

Mutation

G1202R

Promoter

CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

Plasmid

#169161

Purpose

To express nsp10-nsp14 fusion protein in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab Synthetic, SARS-CoV-2)

Tags

14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp2-a

Plasmid

#40047

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pDest27-mBIN1+13+17

Plasmid

#61097

Purpose

Express N-terminal GST tagged-mouse BIN1+13+17 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pTWIN1-His6-Ssp-Httex1-43Q

Plasmid

#84352

Purpose

Expression of the human Huntingtin Exon1 protein containing 43Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B5

Plasmid

#172846

Purpose

CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1 hSlp2-a

Plasmid

#40056

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a (SYTL2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q

Plasmid

#84349

Purpose

Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37b, 46]

Plasmid

#26570

Depositor

Diane Lipscombe

Article

Lin et al Neuron. 1997 Jan . 18(1):153-66.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 24a. Contains …

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pT7-V5-SBP-C1-HsRRP41

Plasmid

#64871

Purpose

expresses V5- and SBP-tagged RRp41 in mamalian cells

Depositor

Elisa Izaurralde

Insert

RRP41 (EXOSC4 Human)

Tags

V5 and SBP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46]

Plasmid

#26571

Depositor

Diane Lipscombe

Article

Lin et al Neuron. 1997 Jan . 18(1):153-66.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 31a Contains e…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsXRN1_K

Plasmid

#146784

Purpose

Mammalian Expression of HsXRN1

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsXRN1 (XRN1 Human)

Expression

Mammalian

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B1

Plasmid

#172842

Purpose

CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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VAMP2-SNAP

Plasmid

#105288

Purpose

VAMP2 tagged with SNAP

Depositor

Justin Taraska

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

VAMP2-SNAP (Vamp2 Rat)

Tags

SNAP tag

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4DBD-Hsp70

Plasmid

#62903

Purpose

Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4DBD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 CMV 3xFG eIF4G1 +MIC

Plasmid

#158776

Purpose

pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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VAMP2-pHmScarlet0

Plasmid

#166891

Purpose

For vesicle exocytosis visualization using pHmScarlet0

Depositor

Pingyong Xu

Article

Liu et al Nat Commun. 2021 Mar 3;12(1):1413. doi:

Insert

VAMP2 (Vamp2 Mouse)

Tags

pHmScarlet0

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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