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pHyg-AID(31-114)-9myc

Plasmid

#99517

Purpose

Truncated C-terminal AID-9myc degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(31-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-CBD-hGLY

Plasmid

#134596

Purpose

Low Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tag

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

human glycine acyltransferase 2 (GLYATL2 )

Use

Tags

chitin binding domain

Expression

Bacterial

Mutation

Promoter

yibD

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA10_no stop

Plasmid

#83848

Purpose

Gateway donor vector for IAA10 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indoleacetic acid-induced protein 10 (IAA10 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

no stop codon

Promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM17-HA

Plasmid

#65221

Purpose

Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM17 (ADAM17 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 18-473

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pRetroX GFP T2A Cre

Plasmid

#63704

Purpose

Inducible expression of GFP T2A Cre fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

T2A Cre

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFlag IFIT3

Plasmid

#53553

Purpose

mammalian expression of IFIT3

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT3 (IFIT3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pHyg-AID*

Plasmid

#99515

Purpose

C-terminal AID* degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-114) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

Expression

Mutation

internal inversion of nucleotides 265-312 of the …

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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p5xUAS-Flag-NPM1wt

Plasmid

#131822

Purpose

4-hydroxytamoxifen inducible vector expressing Flag-NPM1wildtype (human) in mammalian cells through transduction

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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p5xUAS-Flag-NPM1c

Plasmid

#131823

Purpose

4-hydroxytamoxifen inducible vector expressing Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transduction

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pKan-PRFA1-9myc-AID*(N)

Plasmid

#99528

Purpose

N-terminal 9myc-AID* degron cassette with RFA1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

RFA1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pKan-PCUP1-9myc-AID*(N)

Plasmid

#99527

Purpose

N-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pKan-PCUP1-AID*(N)-9myc

Plasmid

#99526

Purpose

N-terminal AID*-9myc degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB cdc42 QL

Plasmid

#61636

Purpose

mammalian expression of cdc42 constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

Cdc42 (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

Q61L, constitutively active

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/TO-LacR-TopBP1

Plasmid

#31313

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

Insert

TopBP1 (TOPBP1 Human)

Use

Tags

FLAG and LacR

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCDNAIIIB rhoA QL

Plasmid

#61632

Purpose

mammalian expression of rhoA constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

rhoA (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

Q63L, constitutively active

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pET28a(+) 6xHis-TEV-IFIT3

Plasmid

#53559

Purpose

bacterial expression of IFIT3

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT3 (IFIT3 Human)

Use

Tags

6xHIS and TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pTriEX-Antenna-GDI Cdc42

Plasmid

#101681

Purpose

"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Cdc42

Use

Tags

6xHis and FRET Antenna (mCerulean-cpVenus)

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNAIIIB HA JNK

Plasmid

#61630

Purpose

mammalian expression of JNK

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

JNK1 beta1 isoform (MAPK8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
-227 hTF (mutant NF-kB)

Plasmid

#15443

Depositor

Nigel Mackman

Article

Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

NF-kappaB binding domain mutated

Promoter

Available since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
p258 pSP72 hAR-LBD

Plasmid

#12492

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

AR LBD (AR Human)

Use

Tags

Expression

Bacterial

Mutation

AR ligand binding domain

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only