We narrowed to 1,293 results for: Ncl
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pBS_KS_attB2_SA(2)_T2AGAL4
Plasmid#125213PurposeArtificial phase 2 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGAL4
UseOtherTagsT2AAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET3C-ARL16(CO)-His6
Plasmid#216485PurposeUsed for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.DepositorInsertADP-ribosylation factor-like 16 (ARL16 Human)
Tagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionBacterialPromoterT7Available SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-Erk2 donor
Plasmid#113104PurposeDonor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequenceDepositorInsertN terminal Erk2 donor region including homology arms and HaloTag
UseSynthetic BiologyAvailable SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#45
Plasmid#110926PurposeVector for expressing proteins in most or all tissues, including nervous system, under control of the inducible heat shock promoter in C. elegansDepositorInsertshsp-16-2 promoter
unc-54 3' control region
ExpressionBacterialAvailable SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RPL13A-FKBPx2
Plasmid#104473PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx2-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
TagsFKBPx2-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Id2-Halo donor
Plasmid#113116PurposeDonor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequenceDepositorInsertId2 donor region including homology arms and HaloTag
UseSynthetic BiologyAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(0)_T2AGeneSwitch
Plasmid#125214PurposeArtificial phase 0 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGeneSwitch
UseOtherTagsT2AAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLSLGUS
Plasmid#51517PurposepLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmidDepositorInsertsGUS
3kb filler sequence
UsePlant t-dna plasmidTagsNLS and flagPromotervirion-sense LIR and 2x35SAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pTSA-38
Plasmid#17328DepositorInsertstreptavidin
ExpressionBacterialMutationTruncated to include only amino acids 16-133 of m…Available SinceFeb. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLY108
Plasmid#130938PurposeEngineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB1
UseSynthetic BiologyExpressionBacterialPromoterPlux2Available SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDH5
Plasmid#83775PurposeUseful for tagging yeast proteins with C-terminal YFP tag; his selectionDepositorInsertYFP
TagsYFPExpressionYeastMutationS65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…Available SinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gli3ZF in AINGpCAGGS
Plasmid#121972PurposeGli3 zinc fingersDepositorInsertGli3ZF (GLI3 Human)
UseChick electroporationTags5xmycExpressionMammalianMutationN terminus and C terminus deletion only nt 1411-1…Available SinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
DPY-pNCS
Plasmid#17365DepositorInsertMMLV proviral DNA
Mutationdeletion that removes 5 amino acids (DPPPY) from …Available SinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
AAA
Plasmid#78487Purpose2135nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tailDepositorInsertCAN1-LUC
ExpressionBacterialMutationCAN1 contains no stop codonAvailable SinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLY103
Plasmid#130937PurposeEngineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA1
UseSynthetic BiologyExpressionBacterialPromoterPlux2Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RPL13A-FKBPx4
Plasmid#104474PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx4-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
TagsFKBPx4-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP154
Plasmid#106360PurposeA vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr II.DepositorTypeEmpty backboneExpressionWormAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
