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We narrowed to 9,671 results for: crispr plasmids

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Showing: 661 - 680 of 9671 results

  1. pTHRB.1.0-gDNA

    Plasmid
    #112429
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor THRB
    Depositor
    Kevin White
    Insert
    THRB (THRB Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112429-plasmid-map-sequence-id-230365

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  2. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83961-plasmid-map-sequence-id-214594

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  3. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226429-plasmid-map-sequence-id-458099

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  4. lenti-dCas9-VP64-BFP

    Plasmid
    #196711
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-VP64-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    196711-plasmid-map-sequence-id-392561

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  5. lenti-UCOE-dCas9-BFP-VP64

    Plasmid
    #196717
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196717-plasmid-map-sequence-id-388038

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  6. pYJ12-PB-U6-ND1-acti-gRNA1

    Plasmid
    #131056
    Purpose
    Activation of NeuroD1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NeuroD1 activation gRNA1 (NEUROD1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131056-plasmid-map-sequence-id-256748

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  7. pYJ13-PB-U6-ND1-acti-gRNA2

    Plasmid
    #131057
    Purpose
    Activation of NeuroD1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NeuroD1 activation gRNA2 (NEUROD1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131057-plasmid-map-sequence-id-256893

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  8. pYJ14-PB-U6-ND1-acti-gRNA3

    Plasmid
    #131058
    Purpose
    Activation of NeuroD1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NeuroD1 activation gRNA3 (NEUROD1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131058-plasmid-map-sequence-id-256735

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  9. pSpec5'

    Plasmid
    #228549
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228549-plasmid-map-sequence-id-454858

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  10. pSpec3'

    Plasmid
    #228548
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228548-plasmid-map-sequence-id-454857

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  11. pdCas9

    Plasmid
    #46569
    Purpose
    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9
    CRISPR array
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46569-plasmid-map-sequence-id-364492

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  13. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

    Plasmid
    #99698
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV
    Depositor
    George Church
    Type
    Empty backbone
    Use
    AAV
    Available Since
    Aug. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    99698-plasmid-map-sequence-id-194602

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  14. p-dCas9-p300-Hygro

    Plasmid
    #104411
    Purpose
    transient expression of dCas9-p300 fusion protein
    Depositor
    Stephan Beck
    Insert
    p300 (EP300 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104411-plasmid-map-sequence-id-204503

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  15. pb-CAGGS-dCas9-VP64-blasto

    Plasmid
    #218556
    Purpose
    Encodes CAG promoter-driven dCas9-VP64 with a bipartite SV40 NLS between dCas9 and VP64, along with blasticidin resistance
    Depositor
    Alejandro Chavez
    Insert
    dCas9
    Use
    CRISPR; Piggybac transposition
    Tags
    2xSV40NLS-VP64
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    218556-plasmid-map-sequence-id-431934

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  16. pVID

    Plasmid
    #236635
    Purpose
    CRISPR cloning, gRNA expression, and detection of undesired vector insertion during HDR for Drosophila
    Depositor
    Brian McCabe
    Type
    Empty backbone
    Use
    CRISPR
    Promoter
    dU6:3
    Available Since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    236635-plasmid-map-sequence-id-469973

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  17. pORFE1052

    Plasmid
    #112078
    Purpose
    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    2x35S
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112078-plasmid-map-sequence-id-217613

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  18. pORFE0014

    Plasmid
    #112076
    Purpose
    AtCAS9 nickase D10A module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A without stop codon
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112076-plasmid-map-sequence-id-217621

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  19. BPK1520_blastR

    Plasmid
    #175289
    Purpose
    Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection
    Depositor
    Ronald Cohn
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV/U6
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    175289-plasmid-map-sequence-id-346344

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  20. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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Showing: 661 - 680 of 9671 results