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Plasmid#201023PurposeBacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertopen reading frame 9c
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His
Plasmid#195610PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tagDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationT825C, C900G, A901C, A903C, T1131G, T1137C, T1227…PromoterT7Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3 del C3-2xStrep
Plasmid#170316PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.DepositorInsertQ22YU3 C-terminal truncation (Delta-C3)
UseTagsStrep-Tag IIExpressionInsectMutationDeletion of C-terminal C3-fingerPromoterpolyhedrinAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 R159K
Plasmid#123626PurposeExpresses GgKDELR2 with Arg159Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertKDELR2 (KDELR2 Chicken)
UseTagsTEV-GFP-HisExpressionYeastMutationArg159LysPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 H12A
Plasmid#123619PurposeExpresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertKDELR2 (KDELR2 Chicken)
UseTagsTEV-GFP-HisExpressionYeastMutationHis12AlaPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 R47A
Plasmid#123620PurposeExpresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertKDELR2 (KDELR2 Chicken)
UseTagsTEV-GFP-HisExpressionYeastMutationArg47AlaPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 R47K
Plasmid#123621PurposeExpresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertKDELR2 (KDELR2 Chicken)
UseTagsTEV-GFP-HisExpressionYeastMutationArg47LysPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 E127A
Plasmid#123622PurposeExpresses GgKDELR2 with Glu127Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertKDELR2 (KDELR2 Chicken)
UseTagsTEV-GFP-HisExpressionYeastMutationGlu127AlaPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 E127Q
Plasmid#123623PurposeExpresses GgKDELR2 with Glu127Gln mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertKDELR2 (KDELR2 Chicken)
UseTagsTEV-GFP-HisExpressionYeastMutationGlu127GlnPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 Y158F
Plasmid#123624PurposeExpresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertKDELR2 (KDELR2 Chicken)
UseTagsTEV-GFP-HisExpressionYeastMutationTyr158PhePromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 R159A
Plasmid#123625PurposeExpresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertKDELR2 (KDELR2 Chicken)
UseTagsTEV-GFP-HisExpressionYeastMutationArg159AlaPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104619PurposeExpresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4DepositorInsert3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
UseTagsFLAGExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3
Plasmid#89975PurposeExpresses GFP-tagged ataxin-3DepositorInsertataxin-3 (ATXN3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-His-hUbi
Plasmid#31815DepositorInsertUbiquitin (UBB Human)
UseTagsHISExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
VPS13C^mClover3
Plasmid#118760PurposeVPS13C internally tagged with mClover3 at residue 1914.DepositorInsertVPS13C (VPS13C Human)
UseTagsmClover3 inserted at position aa 1914ExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
Plasmid#61591PurposeA single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.DepositorInsertshSaCas9
Chimeric guide for SaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
lenti SYN-FLAG-dCas9-VPR
Plasmid#114196PurposeLentivirus compatible dCas9-VPR construct driven by the human SYN promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterSYNAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-YR-ICUE-mGold2s
Plasmid#231789PurposeVisualization of cAMP dynamics using YR-ICUE-mGold2sDepositorInsertYR-ICUE-mGold2s
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v3 (fl)
Plasmid#137814Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v3 (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull length and N280D in the V3 regionPromoterPGKAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only