We narrowed to 5,360 results for: kras

1. pcDNA3-PM-InPAkt

   Plasmid

   #181915

   Purpose

   Indicator of Phosphoinositides using Akt; targeted to the plasma membrane.

   Depositor

   Jin Zhang

   Article

   Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

   Insert

   pm-InPAkt
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Contains the following mutations with respect to …

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rgl2-CAAX-pcw107-V5

   Plasmid

   #64641

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   Rgl2 plus C-term KRAS (Kras Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   C-term of Kras

   Promoter

   PGK

   Available since

   Dec. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pLenti6-GFP

   Plasmid

   #35637

   Depositor

   Daniel Haber

   Article

   Singh et al Cell. 2012 Feb 17;148(4):639-50.

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pSPORT-RREB1

   Plasmid

   #41145

   Depositor

   Joshua Mendell

   Article

   Kent et al Oncogene. 2012 Jul 2. doi: 10.1038/onc.2

   Insert

   RREB-1 (RREB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCas9-P2A-BFP

   Plasmid

   #149447

   Purpose

   Expresses Cas9 and BFP in a lentiviral vector.

   Depositor

   Jonathan Chernoff

   Article

   Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

   Insert

   P2A-BFP
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   In frame with Cas9

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE puro K-Ras V12

   Plasmid

   #9052

   Purpose

   Retroviral expression of a constitutively active form of K-Ras

   Depositor

   William Hahn

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V. Constitutively active.

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PEmax-SpG

   Plasmid

   #211549

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

   Insert

   PEmax-SpG
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRXIP-GFP-9K0Q

   Plasmid

   #221739

   Purpose

   Stable expression of GFP-9K0Q in mammalian cells to monitor membrane charge

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-170

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDest-635

   Plasmid

   #159582

   Purpose

   Gateway Destination vector for protein expression in baculovirus

   Depositor

   Dominic Esposito

   Article

   Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PEmax-SpG-P2A-hMLH1dn

    Plasmid

    #211550

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG-P2A-hMLH1dn
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-CaaX(tK)

    Plasmid

    #109540

    Purpose

    Multisite gateway vector for 3' tagging with the CaaX domain from Kras

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    CaaX domain from Kras
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-pmEKAR4

    Plasmid

    #174440

    Purpose

    Plasma membrane-targeted EKAR4.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    pmEKAR4
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWZL-hygro-FAKWT

    Plasmid

    #216540

    Purpose

    This retroviral plasmid expresses the human wild type PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    wild type sequence

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB_Cdc42-TomKat-sensor-CAAX

    Plasmid

    #172473

    Purpose

    Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.

    Depositor

    Sean R Collins

    Article

    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

    Insert

    tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
    

    Use

    Piggybac transposon

    Tags

    polybasic and CAAX motif from Kras, tdKatushka2, …

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti6-BMPR1A(Q233D)-V5

    Plasmid

    #35638

    Depositor

    Daniel Haber

    Article

    Singh et al Cell. 2012 Feb 17;148(4):639-50.

    Insert

    BMP Receptor Type1A Q233D (BMPR1A Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Glutamine 233 to Aspartate

    Promoter

    CMV

    Available since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pDest-521

    Plasmid

    #159688

    Purpose

    Gateway destination vector for E. coli protein expression

    Depositor

    Dominic Esposito

    Article

    Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWZL-hygro-FAKY925F

    Plasmid

    #216547

    Purpose

    This retroviral plasmid expresses a mutated cDNA (dominant negative: Y925F ) of human PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (Y925F) (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    FAK dominant negative mutation Y925F

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-pmEMBer

    Plasmid

    #174441

    Purpose

    Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    pmEMBer
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWZL-hygro-FAKS722A

    Plasmid

    #216545

    Purpose

    This retroviral plasmid expresses a mutated cDNA (dominant negative: S722A) of human PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (S722A) (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    FAK dominant negative mutation S722A

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWZL-hygro-FAKY861F

    Plasmid

    #216546

    Purpose

    This retroviral plasmid expresses a mutated cDNA (dominant negative: Y861F) of human PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (Y861F) (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    FAK dominant negative mutation Y861F

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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